Nutritional Composition with miR-3184 for Infant Formula

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Solution Overview

Problem

There is a need for nutritional compositions that mimic the natural composition of breast milk, particularly during different stages of lactation, as not all infants have access to natural breast milk, and these compositions should include miRNAs that promote healthy growth and development in infants.

Innovation Solution

A nutritional composition comprising miR-3184, which is present in natural breast milk, is developed, along with additional microRNAs such as let-7d, miR-196a, miR-187, miR-516a, miR-92b, and miR-3126, to mimic the natural miRNA profile in breast milk, suitable for use as an infant formula, fortifier, or supplement, promoting healthy growth and development.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If infant formula is used instead of natural breast milk, then accessibility to nutrition is improved, but the natural miRNA profile and its beneficial effects are lost

Engineering Contradiction:
Improveaccessibility to nutritionVSAvoidnatural miRNA profile
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent creates a copy of the natural breast milk miRNA profile by adding specific microRNAs (including miR-3184, let-7d, miR-196a, miR-187, miR-516a, miR-92b, and miR-3126) to infant formula at concentrations that mimic natural breast milk. This copying approach allows the formula to provide both the accessibility advantage and the natural miRNA profile benefit simultaneously.

Inventive Principle:
Principle #26Copying

2Reliability

If miRNA is added to nutritional composition, then gene expression modulation is improved, but manufacturing complexity increases

Engineering Contradiction:
Improvegene expression modulationVSAvoidmanufacturing complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent specifies precise concentration ranges for multiple microRNAs in the nutritional composition (e.g., miR-3184 at 0.1-10000 pmol/L, let-7d at 0.1-10000 pmol/L, miR-196a at 0.1-10000 pmol/L, miR-187 at 0.1-10000 pmol/L, miR-516a at 0.1-10000 pmol/L, miR-92b at 0.1-10000 pmol/L, and miR-3126 at 0.1-10000 pmol/L). By defining these specific parameter ranges, the patent simplifies manufacturing by providing clear targets for formulation while ensuring reliable gene expression modulation effects.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS20230248042A1Nutritional composition comprising MIR-3184
Publication Date: 2023.08.10 SOCIETE DES PRODUITS NESTLE SA

AI summary

A nutritional composition comprising miR-3184. miR-3184 or said nutritional composition for use as a medicament. Use of miR-3184 to modulate gene expression of one or more gene selected from Lipin 2 or Insulin Like Growth Factor 2. A method of producing said nutritional composition.