Fusion With DNA-Binding Domain TRIZ Cases
- CISH Gene Modification via Modular Nuclease Systems
Modular nuclease segmentation improves genetic modification precision while managing system complexity for reliable cancer therapy.
- Plant Breeding Method for Removing Genetic Linkage Drag
A plant breeding method uses site-specific nucleases to induce targeted recombination events.
- Cas9-Activator Fusion Proteins for Synergistic Gene Expression
A catalytically inactive Cas9 fused to transcriptional activation domains directs guide RNAs to specific genomic loci.
- Fusion Polypeptide Gene Regulation via Cell-Penetrating Peptides
A fusion polypeptide combines cell penetration and DNA binding to activate target genes.
- Daisyfield Gene Drives for Localized CRISPR Activity
Daisyfield gene drives utilize segmented CRISPR chains to prevent global dissemination while enabling efficient multi-locus trait introduction.
- TALEN DNA Editing for Tandem Repeat Deletion
Engineered TALENs target hairpin-forming DNA sequences to delete pathological repeats without off-target mutations.
- dCas9 Fusion Proteins for Frataxin Expression Modulation
Deactivated Cas9 fusion proteins target frataxin regulatory elements to increase gene expression levels.
- Cascade-Deaminase Complex for Base Editing Without DNA Cleavage
A Cascade complex paired with a deaminase enzyme converts targeted nucleotides in host cells without cleaving double-stranded DNA.
- Epigenetic Editing via Endogenous Enzyme Recruitment
Guide RNA directs endogenous epigenetic enzymes to specific gene loci, eliminating toxicity and off-target effects from synthetic repressors.
- Mutant MMLV Reverse Transcriptase for GC-Rich cDNA Synthesis
A mutant MMLV reverse transcriptase synthesizes full-length cDNA from RNA templates.
- Zinc Finger Peptide Synthesis via Fragment Segmentation
Segmenting zinc finger domains into synthesizable fragments bypasses recombinant nucleic acid risks while simplifying library creation.
- Hybrid Zinc Finger Degron Sequences for Low-Dose Protein Control
Hybrid zinc finger degron sequences reduce immunogenicity and side effects by enhancing sensitivity to immunomodulatory drugs at lower concentrations.
- Fusion Protein Gene Expression Control via Small Molecule Dimerization
Small molecule-induced dimerization of fusion proteins reduces background expression while enabling temporal modulation control.
- Programmable AAV Capsid Retargeting via ssDNA-Binding Domain
A programmable adeno-associated virus capsid integrates an ssDNA-binding domain to enable modular antibody attachment for targeted gene delivery.
- Effector Domain Discovery for Synthetic Transcription Factors
High-throughput systems discover effector domains for synthetic transcription factors.
- Cationic Lipid Nanoparticles for Lung Gene Editing
Cationic lipid nanoparticles encapsulate engineered nucleases to penetrate mucus barriers and correct CFTR mutations.
- CRISPR Base Editing CCR5 Gene HIV Protection
CRISPR base editing modifies the CCR5 gene to block HIV entry, avoiding off-target effects and genome instability from traditional nuclease methods.