Daisyfield Gene Drives for Localized CRISPR Activity

The gene drive system is divided into multiple independent loci (at least two repeated regions in the genome) that can be independently manipulated. Each locus contains gene drive elements that target specific repeated genomic sequences, allowing the population to be edited at multiple locations simultaneously while maintaining control over the spread dynamics at each location.

The gene drive elements are designed to target specific repeated genomic sequences (such as ribosomal RNA gene clusters) that are locally concentrated in the genome. By directing the nuclease to cut only at these specific repeated regions, the system achieves localized genetic modification without affecting the entire genome, and the spread can be confined to specific population contexts.

The gene drive elements are designed to recognize and target repeated genomic sequences that occur multiple times throughout the genome (such as ribosomal RNA gene clusters). A single gene drive element can therefore target multiple identical or similar sequences across different chromosomal locations, achieving multi-locus editing with a single designed component rather than requiring separate elements for each target site.

The system utilizes the natural repetition of genomic sequences (such as multiple copies of ribosomal RNA genes) to amplify the effect of the gene drive. By targeting these naturally occurring repeated sequences, the system leverages the existing genomic architecture to achieve widespread trait introduction without requiring artificial duplication of the entire gene drive construct at multiple locations.