Fusion With RNA-Binding Domain TRIZ Cases
- Extended Guide RNA Editing for Broader Plant Allele Replacement
Extended guide RNA with CRISPR effectors and reverse transcriptase broadens plant allele editing beyond base editor PAM and residue limits.
- RNA-Encoded DNA Replacement for Broader Allele Editing
A Type V CRISPR-Cas, reverse transcriptase, and extended guide RNA expand allele editing beyond conventional base changes in plants.
- RNA-Guided CRISPR Effectors for Specific RNA Targeting
Guide RNA-mediated CRISPR effectors improve sequence-specific RNA targeting while limiting off-target effects for editing, knockdown, and diagnostics.
- Self-Diversifying Polynucleotides for High-Diversity Molecular Libraries
Error-prone reverse transcription mutates a variable region to generate diversified molecular libraries with high efficiency for binding partner screening.
- Fusion Polypeptide Architecture for Sustained Gene Expression Control
Linked Cas and epigenetic regulator domains enable precise target binding with sustained gene expression changes lasting over 30 days.
- Genome Editing Composition With Cas-CLOVER for Precise Targeted Integration
Mutant Cas-CLOVER, guide RNA, and lipid nanoparticles improve targeted genome integration yield while reducing unintended edits for cell therapy.
- Chimeric RNA Editing Proteins for Translation Rate Sequencing
RNA-editing chimeric proteins label target mRNA for sequencing-based translation rate measurement without ribosomal RNA removal.
- Trans-Splicing Molecules for AAV-Limited ABCA4 and CEP290 Repair
Segmented trans-splicing molecules overcome AAV size limits to repair ABCA4 and CEP290 mutations linked to Stargardt disease and LCA10.
- Trafficked RNA Barcodes for Mapping Synaptic Connectivity
RNA barcodes and fusion-protein trafficking link synaptic connectivity with single-cell transcriptomics for scalable, minimally invasive neuroanatomy mapping.
- Multiplex Guide Polynucleotide Expression Using RNase III Cleavage
RNase III cleavage of stem-loop transcripts enables single-delivery expression of multiple guide polynucleotides for plant genome editing.
- LIDAR RNA Editing Receptors for Ligand-Triggered Polypeptide Expression
Ligand-induced dimerization recruits ADAR to output RNA, enabling post-transcriptional control of polypeptide expression with targeted specificity.
- Mutant RID Fusion Vectors for Soluble Norovirus VLP Expression
Targeted RID mutations improve VP1 soluble expression and folding in E. coli, enabling faster, more uniform multigenotype norovirus VLP production.
- Trans-Splicing RNA Tethering for Efficient Exon Replacement
Tethering proteins and engineered small nuclear RNAs promote exon insertion into target RNA, improving genetic defect correction without CRISPR.