Fusion With RNA-Binding Domain TRIZ Cases

Extended guide RNA with CRISPR effectors and reverse transcriptase broadens plant allele editing beyond base editor PAM and residue limits.

A Type V CRISPR-Cas, reverse transcriptase, and extended guide RNA expand allele editing beyond conventional base changes in plants.

Guide RNA-mediated CRISPR effectors improve sequence-specific RNA targeting while limiting off-target effects for editing, knockdown, and diagnostics.

Error-prone reverse transcription mutates a variable region to generate diversified molecular libraries with high efficiency for binding partner screening.

Linked Cas and epigenetic regulator domains enable precise target binding with sustained gene expression changes lasting over 30 days.

Mutant Cas-CLOVER, guide RNA, and lipid nanoparticles improve targeted genome integration yield while reducing unintended edits for cell therapy.

RNA-editing chimeric proteins label target mRNA for sequencing-based translation rate measurement without ribosomal RNA removal.

Segmented trans-splicing molecules overcome AAV size limits to repair ABCA4 and CEP290 mutations linked to Stargardt disease and LCA10.

RNA barcodes and fusion-protein trafficking link synaptic connectivity with single-cell transcriptomics for scalable, minimally invasive neuroanatomy mapping.

RNase III cleavage of stem-loop transcripts enables single-delivery expression of multiple guide polynucleotides for plant genome editing.

Ligand-induced dimerization recruits ADAR to output RNA, enabling post-transcriptional control of polypeptide expression with targeted specificity.

Targeted RID mutations improve VP1 soluble expression and folding in E. coli, enabling faster, more uniform multigenotype norovirus VLP production.

Tethering proteins and engineered small nuclear RNAs promote exon insertion into target RNA, improving genetic defect correction without CRISPR.

A G-quadruplex aptamer blocks polymerase at low temperature and releases it during heating to suppress non-specific isothermal amplification.

Site-specific DNA-binding agents disrupt anchor-sequence conjunctions to enhance or suppress transcription in defective gene regulation.

Site-specific DNA-binding agents disrupt anchor-sequence conjunctions by competing with endogenous polypeptides to precisely modulate transcription.

Compact engineered effectors combine selected activation domains to preserve strong CRISPR-linked gene regulation while improving viral packaging.

Fusion proteins pair dsRNA sensing with RNA-cleaving effector domains to block diverse viruses beyond narrow virus-specific strategies.

A HEPN-domain endonuclease improves gene editing specificity and efficiency while easing PAM and packaging limits in nucleic acid modification.

Fusion spike polypeptides with heterologous tags sustain high anti-spike IgG and variant neutralization without repeated boosters.

Cas13e and Cas13f retain guide-directed RNA targeting in compact proteins suited to AAV packaging while limiting collateral RNase activity.

Engineered sgRNAs recruit fluorescent proteins through RNA aptamers to image single-copy genomic loci with higher resolution and lower background.

Guide RNA separates locus recognition from Cas-protein cleavage, reducing protein engineering while enabling precise targeting of supercoiled DNA.

Compact AAV delivery uses trans-splicing molecules to correct large ABCA4 and CEP290 mutations without packaging full gene sequences.

Cas13-guided RDRP uses an RpegRNA template to add precise nucleotide sequences at the 3′ end of target RNA without large enzyme amounts.

A sequence-recognition module directs an RNA enzyme to mutant mRNA, converting uridine to cytidine for nonsense-mutation correction and full-length protein synthesis.

Template-binding domains bring donor nucleic acids close to Cas9-induced breaks, increasing HDR frequency and editing precision.

Human-derived programmable proteins target RNA and deliver effector domains site-selectively without activating an immune response.

Guide RNAs position dCas13 and deaminases on YAP1 or TAZ mRNA to improve editing precision while limiting off-target effects.

A fused, thermostable group II intron reverse transcriptase counters RNA structure and low fidelity during high-temperature DNA synthesis.

This case uses reusable CRISPR-Cas targeting and adenine deaminase fusions to simplify precise A:T-to-G:C DNA editing.

RNA-regulated destabilization domains link aptamer binding to genetic fluorescence, reducing nonspecific activation in live-cell imaging.

An RBP and gene-targeting agent bind near a poly(A) site to regulate APA, protect target RNA, and enhance protein synthesis.

This case combines RNA-guided DNA binding with adenine deamination to enable targeted A>G editing without double-strand cleavage.

Modified Cas7, Cas5, and Cas6 Cascade complexes use guide RNA recognition for precise nucleic acid detection and gene modification.

Guide RNA directs modified Cascade complexes to target sequences for precise nucleic acid cleavage, visualization, and gene regulation.

HEPN domain engineering addresses off-target activity and PAM limits, enabling more precise gene editing and delivery.

Fusion polypeptide construct combines RNase H-like domain with nucleic acid unwinding enzyme to resolve genome editing specificity and efficiency trade-offs.

Inserting RNA binding domains into the HEPN1 loop of LwaCas13a enhances collateral cleavage activity for nucleic acid sensing.

Multimeric ribonucleoproteins deliver heterologous polypeptides via spontaneous yeast-based auto-assembly.

Engineered donor cells transfer membrane agents and cargo to acceptor cells via receptor interaction.

A fusion protein combines a DNA exonuclease with an RNA binding domain to enable precise genetic modification.

Nuclear-encoded RNA intermediaries mediate genetic transfer to plant plastids, resolving erratic delivery and low efficiency in heterologous protein production.

A chimeric enzyme merges RNA polymerase with capping domains to produce capped mRNA transcripts directly.

Modified DNA probes with biotin labels enhance signal-to-noise ratios, enabling practical detection of single-copy disease-relevant sites.

Supercharged proteins associate with cationic lipids to transport functional nucleases into mammalian cells.

Modified Cas endonucleases optimize cleavage efficiency and specificity for diverse genetic editing tasks.

Cysteine-to-serine mutations in hnRNP-E1 enable homocysteine-independent mRNA binding, resolving physiological condition constraints.

Replacing viral domains with human protein segments boosts transactivation while improving cellular tolerance in clinical applications.

A chimeric protein fuses a PSMA targeting moiety with a dsRNA binding domain to deliver genetic cargo.

Measuring SLBP and specific histones replaces standard viral load tests to predict disease progression and guide targeted therapeutic interventions.

A retroviral particle encapsidates multiple non-viral RNAs via Gag polyprotein binding domains for simultaneous cellular delivery.