Self-Diversifying Polynucleotides for High-Diversity Molecular Libraries
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Solution Overview
Problem
Existing systems lack efficiency and diversity in generating libraries of diversified molecules for screening novel binding partners for antigens and pathogens.
Innovation Solution
A self-diversifying system comprising a polynucleotide encoding one or more open reading frames, including variable and template regions, with a reverse transcriptase, capable of generating a library of diversified molecules with at least 80% efficiency by incorporating mutations into the variable region.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional systems are used to generate diversified molecules, then the process can be performed, but the efficiency is low and the degree of diversity is insufficient
Solution Approach 1:
The system employs self-diversifying elements that automatically generate diversity through their own molecular mechanisms. The polynucleotide sequences encode for reverse transcriptase enzymes that inherently introduce mutations during reverse transcription, creating diversified molecules without requiring external intervention or complex artificial systems. This self-service mechanism achieves both high efficiency and sufficient diversity.
Solution Approach 2:
The invention utilizes error-prone reverse transcription as a parameter change mechanism. By controlling the fidelity of the reverse transcriptase enzyme, the system achieves desired levels of diversity in the generated molecules. The polynucleotide sequences are designed to produce specific mutation rates and types, allowing efficient generation of diversified molecules with controlled variability.
2Adaptability or versatility
If high diversity is achieved, then the library contains sufficient varied molecules, but the generation efficiency decreases
Solution Approach 1:
The self-diversifying elements perform the diversity generation function intrinsically through their molecular structure and enzymatic activity. The reverse transcriptase enzymes encoded by the polynucleotides automatically introduce mutations during the reverse transcription process, eliminating the need for separate diversification steps and maintaining high generation efficiency while achieving sufficient diversity.
Solution Approach 2:
The system maintains continuous diversity generation through the ongoing reverse transcription process. The polynucleotide sequences are designed to continuously produce diversified molecules as they are synthesized, rather than requiring discrete diversification steps. This continuous action ensures both high efficiency and adequate diversity in the generated library.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system effectively generates a diverse library of molecules with high efficiency, enabling rapid identification of binding partners for antigens and pathogens through methods like phage display.
Implementation Method 1
the system is configured to generate a library of diversified molecules with an efficiency of at least 80% as measured by a number of the diversified molecules in a total number of molecules in the library
Implementation Method 2
capable of generating a library of diversified molecules with at least 80% efficiency by incorporating mutations into the variable region
Data Source
AI summary
A diversity-generating system comprising: a polynucleotide comprising: a variable region within a sequence encoding a protein or polypeptide, wherein the system is configured to generate a library of diversified molecules with an efficiency of 80% as measured by number of the diversified molecules in total number of molecules. The system may further comprise a template region; and a reverse transcriptase or functional domain thereof.