Hydrolases TRIZ Cases

Steric hindrance limits large lysine derivative insertion; localized enzyme mutations broaden substrate recognition and increase unnatural amino acid incorporation.

Prime editing efficiency is limited by mismatch repair; siRNAs and ASOs targeting MSH2, PMS2, MSH6, and MLH1 suppress this pathway.

A paired single-stranded nucleic acid complex forms transposase binding sites for site-specific, PAM-independent editing beyond CRISPR sequence-size limits.

Down-regulating miR528 with CRISPR/Cas9 or STTM increases tillering and regeneration in gramineous forage grasses.

Random-locus editing can silence transgenes during PSC differentiation; CISH or Rosa26 integration supports stable, uniform expression in derived iNK cells.

See how modular PEgRNAs combine target recognition, reverse-transcription priming, and stabilizing 3′ motifs to improve DNA editing efficiency.

A CM1 ubiquitin variant binds the 53BP1 Tudor domain to disrupt H2A interaction and improve HDR in CRISPR editing.

Individual compartments pair enzyme variants with DNA/RNA targets, and sequencing quantifies activity across large libraries.

Early blood testing of UCH-L1 and GFAP addresses weak mild-TBI detection and supports objective severity assessment within two hours.

An integrated helicase-nanopore unwinds double-stranded DNA before translocation, improving single-base sensing without external enzymes.

Extreme Cas-protein diversity can hide new systems; a Cas1-anchored pipeline screens nearby ORFs and locus structure for novel Class 2 effectors.

Extremophilic enzymes depolymerize post-consumer PHB and repolymerize hydroxybutyrate in one cycle, reducing contamination risks.

CMS in triticale 343CMS prevents self-pollination and supports controlled hybrid seed production with restorer lines.

ITR-adjacent transcription termination helps recombinant viral vectors limit read-through and off-target gene expression while preserving intended expression.

An engineered CRISPR-associated transposon combines RNA-guided targeting with transposon integration to avoid DNA double-strand breaks.

A conserved rKLi8.3 antigen enables sensitive, specific Leishmania antibody testing across strains in point-of-care formats.

Modified Cas9 variants stabilize short sgRNA complexes for efficient DNA cleavage in living cells with reduced off-target effects.

Variant acyl-ACP thioesterases help transgenic sorghum accumulate fatty acids and triacylglycerols in leaves and stems for oil feedstocks.

Fluorescent tau biosensor cells and guide RNA libraries reveal genes that enhance or inhibit tau aggregation after seeding.

Engineered guide RNAs direct one Cas enzyme to multiple genomic sites, reducing the cost and complexity of precise genome editing.

Pre-integrated safe-harbor landing sites and SSR recombination enable targeted multi-transgene insertion with fewer cell selections.

CRISPR-based editing of synovial cells lowers IL-1α and IL-1β expression to curb inflammation and support longer-term joint function.

Traditional microbial hosts yield only 1–2 g·L−1, while engineered S. marcescens reaches 40.72 g·L−1 linalool in a 30 L fermenter.

Base editor fusion proteins with splice-site guide RNAs enable precise immune-cell gene disruption while reducing double-strand-break toxicity and off-target effects.

Low-yield corrective sequence insertion is addressed with single-stranded donors and inhibitors that favor HDR in edited cells.

Freeze-thaw cycles, enzymes, surfactant, and endonuclease remove cells and DNA while preserving collagen, elastin, and matrix structure.

A CENH3-mutant CMS haploid inducer transfers a desired nuclear genome into CMS cytoplasm in one cross, bypassing lengthy introgression.

Magnetic beads coated with chitin-binding protein enrich fungal cells before RPA-CRISPR/Cas12a detection within 120 minutes.

TwinPE struggles with DNA inserts above 100 bp; dual-PBS pegRNA template jumping improves efficiency without double-strand breaks.

Genomic barcodes support pooled engineered-cell production while enabling precise variant identification, isolation, and validation.

Temporal vectors encode sequential multiplex genetic events to direct differentiation and control cell states beyond large-scale cultures.

CRISPR/Cas9 disruption of the CGS MTO1 regulatory region removes feedback inhibition, raising seed methionine and protein content.

CRISPR-Cas9 and homologous recombination insert gene-drive constructs into DNA viruses for transmission through asexual viral populations.

Elevated magnesium in IVT reactions reduces dsRNA by-products while preserving RNA yield and integrity without added purification.

Engineered BrCas12b variants maintain activity at 60–70°C, combining isothermal amplification and CRISPR detection in one reaction vessel.

A targeted 12-base-pair deletion lowers myostatin expression, supporting greater muscle mass and low-fat meat without conventional health side effects.

Two sequential PCR approaches use DpnI digestion and two primers to produce accurate mutations in large plasmids within one day.

Systematic amino acid substitutions tune CRISPR nuclease variants for stronger RNA-guide binding, stability, and target specificity.

Deleting selected proteases such as ClpP and Lon helps bacterial hosts protect unstable recombinant proteins and raise yields.

Wild-type proteases lack sufficient PLA activity; targeted amino acid substitutions raise polyester degradation by 5% to 500%.

Anchoring the second-strand primer to the sequencing chip and using Bst3.0 stabilizes synthesis for improved DNB sequencing quality.

Enzymatic pretreatment with glutaminase and protease suppresses dull color formation in textured plant protein, improving brightness without hydrogen peroxide.

Double-strand breaks can cause large deletions in DMD editing; Cas9 nickase–deaminase fusions enable targeted base correction.

Low transformation and regeneration rates in wheat and other crops are addressed with WUS, BBM, and SERK constructs that promote cell division.

Guide RNAs direct Cas9 to excise non-essential dystrophin exons in vivo, producing a truncated protein that retains functional activity.

This fusion combines locus targeting with error-prone polymerase activity to broaden editing windows and directed-evolution sequence space.

Reducing Lox3 expression through silencing or genome editing can improve maize and oilseed rape tolerance to armyworm and selected fungi.

Traditional homologous recombination is labor-intensive and hard to scale; protected templates with Cas guidance improve on-target repair and limit off-site integration.

CRISPR-Cas9 excises CD163 exon 7 and introduces a stop codon, blocking PRRSv entry despite the virus's genetic diversity.

A Pol-II promoter and modular sgRNA let one Cas nuclease target multiple sites, addressing costly redesign and scaling limits in non-conventional yeast.