Engineered Serratia marcescens Strain for Terpenoid Production
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Solution Overview
Problem
Existing methods for producing terpenoids in microorganisms like Escherichia coli and Saccharomyces cerevisiae yield only 1-2 g·L−1, which is insufficient for industrial demands due to high market demand.
Innovation Solution
A method using the mevalonate (MVA) pathway in Serratia marcescens to produce terpenoids, employing an engineered strain of S. marcescens that expresses specific enzymes such as acetyl-CoA acetyltransferase, HMG-CoA synthase, and mevalonate kinase, along with terpene synthases to enhance terpenoid production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional host cells (E. coli and S. cerevisiae) are used for terpenoid production, then the production system is well-established and easy to operate, but the yield is limited to 1-2 g·L−1 which cannot meet industrial demands
Solution Approach 1:
The patent changes the host cell parameter from traditional E. coli or S. cerevisiae to Serratia marcescens, which has different metabolic characteristics and higher tolerance to organic solvents. This parameter change enables achieving yields of 40.72 g·L−1 for linalool, representing a 20-fold increase while maintaining a manageable expression system
Solution Approach 2:
The patent segments the terpenoid production pathway into multiple independent gene expressions (acetyl-CoA acetyltransferase, HMG-CoA synthase, HMG-CoA reductase, mevalonate kinase, phosphomevalonate kinase, and mevalonate pyrophosphate decarboxylase), allowing optimized expression of each enzyme in the MVA pathway to achieve high overall productivity
2Productivity
If the MVA pathway is engineered in Serratia marcescens to achieve high yield, then terpenoid productivity increases to 40.72 g·L−1, but the strain construction and optimization process becomes more complex
Solution Approach 1:
The patent utilizes the multi-functionality of the MVA pathway enzymes in Serratia marcescens, where the same pathway can produce various terpenoids (monoterpenes, sesquiterpenes, diterpenes) by simply changing the downstream synthase genes, making the system universally applicable for different terpenoid production without re-engineering the core pathway
Solution Approach 2:
Serratia marcescens naturally possesses high tolerance to organic solvents and favorable metabolic characteristics that self-support high-level terpenoid production. The strain's inherent properties (solvent tolerance, metabolic flux) serve the production goal without requiring extensive external intervention or complex control systems
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The engineered S. marcescens strain achieves yields of up to 40.72 g·L−1 of linalool in a 30 L fermenter and significant yields of various terpenes like isoprene, 1,8-cineole, and α-pinene, surpassing previous yields and meeting industrial requirements.
Implementation Method 1
Serratia marcescens (S. marcescens) HBQA7 is used as a chassis cell to expresses acetyl-CoA acetyltransferase, HMG-CoA synthase, HMG-CoA reductase, mevalonate kinase, phosphomevalonate kinase, and mevalonate pyrophosphate decarboxylase
Data Source
AI summary
Disclosed are production of terpenoid compound and the strain used by, which belong to the technical field of bioengineering. The disclosure constructs an engineered strain of Serratia marcescens in production of hemiterpenes or monoterpenes, and the engineered strain of S. marcescens can produce linalool, isoprene, isopentenol, 1,8-cineole, α-pinene, pinene, γ-terpinene, geraniol, (+)-limonene, (−)-limonene, myrcene, β-ocimene, sabinene, (−)-α-bisabolol, farnesol, longifolene, valencene, β-elemene, farnesene, patchoulol, pentalenene, and α-santalene. In a 30 L fermenter, the yield of linalool produced by the engineered strain of S. marcescens is 40.72 g·L−1.