Hydrolases TRIZ Cases

Reducing ROS1a DNA glycosylase activity thickens the aleurone layer without pleiotropic effects or agronomic compromise.

Replacing anionic groups with zwitterionic units enables nanodisc stability in low pH and polyvalent cation environments.

A unified enzyme mixture merges linearization and primer removal steps, cutting sequencing time while maintaining read quality.

Unnatural amino acid conjugation maintains RNP complex stability and affinity, resolving low homologous recombination efficiency in SpCas9 and AsCas12a systems.

Chemically modified nucleic acids regulate Cpf1 cleavage activity to balance gene editing efficiency with reduced side effects.

Engineered LbCas12a nuclease variants detect single-stranded DNA targets directly without pre-amplification steps.

A fusion protein recruits the 26S proteasome via a vWA domain to degrade target proteins without ubiquitination.

A Cre-LoxP nucleic acid construct uses TATA-LoxTC9 recombination to enable single sgRNA expression per cell.

Specific bridge helix modifications in Cas12a and Cas12b variants improve DNA cleavage selectivity, resolving precision challenges in gene editing applications.

Optical mapping visualizes fluorescent labels on stretched DNA molecules to quantify repeat arrays and methylation status, bypassing NGS fragmentation limits.

Thermostable phytase removes phytic acid from grain slurry, eliminating pH adjustment steps and ion contamination while maintaining enzyme stability.

Recombinant microorganisms convert vegetable oil fatty acids into polyhydroxyfatty acids via enzymatic oxidation and polymerization.

An aqueous composition stabilizes nucleic acids in saliva samples at room temperature for direct amplification.

Activating endogenous activation-induced cytidine deaminase in B lymphocytes enables precise genome editing without exogenous nucleases.

A BRET biosensor quantifies endonuclease restriction efficiency via luciferase and GFP2 energy transfer signals.

CRISPR-Cas9 nucleases introduce precise double-strand breaks in rice genomes, enabling efficient allele stacking without unwanted genetic variations.

A modified single-guide RNA with internal anchors binds donor DNA segments to improve gene editing precision.

CRISPR tools correct mutations in primary human muscle stem cells, eliminating immunosuppression needs and off-target risks for muscular dystrophy therapy.

A single cell assay uses TALENs to induce double-stranded breaks in chromosomal contacts within gene loops.

A magnetic chemiluminescence immunoassay kit uses a bifunctional fusion protein to detect mycotoxins directly in edible oil samples.

A transposome-mediated tagmentation method uses extension primers to generate tagged fragments for downstream sequencing applications.

Merging OsDN-DTP12, OsSSL13, and four other polynucleotides into a single construct overcomes insufficient drought resistance in crops.

Pre-assembled ribonucleoprotein complexes direct Cas9 enzymes to specific DNA sites for simultaneous multiplex editing.

Maintains over 90% protease activity while destroying amylase through precise pH and temperature control, resolving selectivity-recovery trade-offs.

GH5 polypeptides degrade xanthan gum, reducing the number of enzymatic activities required for complete cleaning.

A cleaning composition merges DNase and hexosaminidase enzymes to degrade biofilm components on textiles.

Immobilized cycloaliphatic peptide acyltransferase adsorbs onto porous inorganic carriers to enable enzyme reuse and enhance stability.

CRISPR-edited NK cells knockout inhibitory receptors to overcome tumor suppression and improve survival.

Engineered hST6Gal-I variants overcome low expression yields by enabling controlled CMP-dependent hydrolysis of terminal sialic acid residues.