Multi-Gene Recombinant Constructs for Drought Tolerance

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Solution Overview

Problem

Current methods for improving drought tolerance in crops are limited, and there is a need for new compositions and methods to enhance drought resistance, as drought stress causes significant yield losses in agricultural crops worldwide.

Innovation Solution

The development of recombinant DNA constructs and modified plants with increased expression of specific polynucleotides encoding drought tolerance proteins, such as OsDN-DTP12, OsSSL13, truncated OsGDSL, OsDN-DTP9, OsWD40-42, and OsABCB12, which are introduced into plants using techniques like CRISPR-Cas or zinc finger nucleases to enhance drought tolerance and grain yield.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding and transgenic approaches are used to improve drought tolerance, then some drought resistance is achieved, but the tolerance level remains insufficient for severe drought conditions

Engineering Contradiction:
Improvedrought toleranceVSAvoidyield loss under drought
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent combines multiple drought tolerance genes (OsDN-DTP12, OsSSL13, truncated OsGDSL, OsDN-DTP9, OsWD40-42, and OsABCB12) into a single recombinant DNA construct, creating a multi-gene system that provides synergistic drought protection beyond what single genes or traditional breeding can achieve

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The invention creates a composite genetic system by integrating six different drought tolerance genes into one construct, analogous to composite materials in engineering, where multiple components work together to provide enhanced performance against drought stress

Inventive Principle:
Principle #40Composite materials

2Reliability

If multiple drought tolerance genes are introduced into plants, then drought resistance is enhanced, but the complexity of genetic modification increases

Engineering Contradiction:
Improvedrought toleranceVSAvoidgenetic construct complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

Multiple drought tolerance genes are merged into a single recombinant DNA construct with shared regulatory elements and selection markers, reducing the number of separate transformation events needed while maintaining enhanced drought protection

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The recombinant DNA construct is designed to be universally applicable across different plant species and drought conditions, with multiple genes providing redundant and complementary functions that enhance overall drought tolerance through various physiological mechanisms

Inventive Principle:
Principle #6Universality (Multi-functionality)

Data Source

PatentUS11371049B2Abiotic stress tolerant plants and polynucleotides to improve abiotic stress and methods
Publication Date: 2022.06.28 SINOBIOWAY BIO AGRI GRP CO LTD

AI summary

Isolated polynucleotides and polypeptides, and recombinant DNA constructs are useful for conferring improved drought tolerance. Compositions (such as plants or seeds) comprising these recombinant DNA constructs; and methods utilizing these recombinant DNA constructs. The recombinant DNA constructs comprise a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotides encode drought tolerance polypeptides.