Hydrolases TRIZ Cases

Replacing porcine pancreas enzymes with microbial phospholipase A2 eliminates animal-derived components while maintaining phospholipid removal effectiveness.

Nuclease polypeptides degrade biofilm DNA and proteins, preventing soil redeposition during mixed laundry loads.

Cas12a enzyme detects target DNA sequences directly using a reporter molecule and filter system.

Segmented mRNA purification and targeted cleavage remove target-irrelevant sequences, reducing sequencing library complexity.

A synthetic polynucleotide expression cassette encodes human propionyl-CoA carboxylase alpha to restore enzyme function in gene therapy constructs.

Mechanical chopping of tissue fragments enables rapid fixing reagent penetration, preventing cellular degradation during dissociation.

Covalent crosslinking locks helicase domains to enhance unwinding activity and force resistance for sequencing applications.

Sequential cleavage by two distinct enzymes ensures precise cargo delivery while preventing premature degradation in non-specific environments.

A microparticle carries an analyte directly into a transmembrane pore for electrical detection.

Suppression DNA constructs decrease stress-sensitive gene expression, increasing grain yield and nitrogen use efficiency under abiotic stress conditions.

Recombinant cyanobacteria convert carbon dioxide into erythritol using sunlight energy.

A restriction enzyme-based DNA library preparation method simplifies genomic sequencing workflows.

Transient nuclease delivery enables precise site-directed modification in plant cells without stable DNA integration.

Segmenting detection to specific ADRβ2 pathway genes improves measurement precision for retinopathy of prematurity without increasing testing complexity.

Engineered Escherichia coli converts depolymerized oil palm empty fruit bunch lignin into adipic acid and levulinic acid via a nine-enzyme metabolic pathway.

Knocking out CRL5 complex genes in hematopoietic cells overcomes tumor microenvironment exhaustion and improves anti-tumor immunity.

CRISPR-Cas mechanisms enable rapid isothermal amplification by eliminating ATP requirements and temperature cycling constraints.

Segmenting detection sites eliminates calibration requirements, enabling precise single-molecule identification.

Fusing a lipase signal sequence to bovine trypsinogen prevents autocatalytic degradation, enabling high-yield production of stable recombinant enzyme.

D236N and D272N mutations enhance thermostability, allowing repeated use at 65°C to resolve enzyme inactivation during oil degumming.

Protease-activated constructs detect pathogens via enzymatic cleavage, avoiding broad-spectrum antibiotic harm.

Targeted substitutions at positions N144 and S224 boost protease catalytic activity by 160%, resolving low-temperature storage stability trade-offs.

Metal ions accelerate lipase-catalyzed hydrolysis, reducing reaction time from 36 hours to two hours while maintaining low by-product levels.

Targeted genome editing of the 7-DR2 enzyme increases provitamin D3 accumulation in tomatoes while maintaining cholesterol biosynthesis stability.

Segmenting CRISPR/Cas components into distinct functional modules resolves the trade-off between editing capability and phenotypic control precision.

Modifying PtrhAT DNA binding activity to alter lignin content and sugar release in genetically modified plants.

Gene editing removes immunogenic antigens from donor cells, resolving immune rejection barriers during universal transplantation.

Recombinant allergen conjugates detect functional IgE on basophils, resolving the trade-off between minimal invasiveness and measurement precision.

Chimeric genes encode enzymes converting herbicides to non-phytotoxic forms, resolving land use inefficiency in hybrid seed production.

Engineered mutant filamentous fungal cells increase polypeptide productivity through modified transcription factor genes.

Plastid selectable markers resolve limited nuclear marker availability by enabling robust marker recycling and reducing regulatory compliance issues.

Multi-point calibration corrects amperometric sensor readings for variable enzyme modulators, resolving accuracy trade-offs in creatinine measurement.

Integrating RecBCD or AddAB repair enzymes alongside Cas9 reduces off-target effects while enhancing homology-directed repair efficiency.

PASEA enriches rare mutant alleles via CRISPR-Cas9 cleavage and isothermal amplification, bypassing deep sequencing.

Engineered zinc finger proteins introduce targeted DNA breaks to replace laborious homologous recombination, enabling rapid cell line generation.

Site-directed mutations in E. coli phytase improve thermostability and proteolytic resistance, enabling phosphate release during high-temperature pelleting.

An engineered nuclease system combines RuvC_III and HNH domains to target DNA sequences with high precision.

Segmented probe amplification captures and detects multiple target particles simultaneously.

A T14 peptide blood test measures biomarker levels to determine Alzheimer's disease pathology stages in living patients.

Enzymatic deglycosylation enhances antibody recognition of PD-L1 proteins for improved detection sensitivity.

Nickase Cas9 gene drive reduces off-target effects and resistant alleles by introducing single-strand DNA nicks instead of double-strand breaks.

A gene detection device uses gold nanoparticles and DNase to enable rapid quantitative analysis without thermal cyclers.

Addamer-based enzymatic synthesis assembles nucleic acids using restriction enzymes and ligases, eliminating phosphoramidite waste.

Segmented CRISPR components with optimized 17-20 nucleotide guide sequences resolve delivery complexity while ensuring mutant allele specificity.

Engineered meganucleases insert chimeric antigen receptors into the human T cell receptor alpha constant region to disrupt endogenous expression.

Phosphatase degrades CMP to enable one-pot synthesis of tetraantennary N-type sugar chains.

Lamiaceae enzymes replace petrochemical routes to produce diterpenes, resolving economic sustainability and industrial efficiency trade-offs.

Polyhydroxy stabilizers maintain enzyme activity for weeks at 37°C, preventing precipitation and eliminating the need for complex surfactant additives.

Mutating the PAM-interacting domain of Cas9 alters sequence specificity, reducing off-target effects while maintaining high editing accuracy.