Amperometric Creatinine Sensor Calibration Modulation Correction
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for calibrating creatinine and creatine measuring devices are inaccurate due to the presence of enzyme modulators in samples, which can vary unpredictably, making it difficult to prepare a calibration solution that matches the modulation level of the target sample.
Innovation Solution
A method using two calibration solutions with different amounts of enzyme modulators to determine the sensitivity of the device, calculating the degree of modulation for each solution, and adjusting the sensitivity to account for the modulation level in the sample, allowing for accurate measurement of creatinine and creatine concentrations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If a single calibration solution with average enzyme modulator concentration is used, then the calibration method is simple and easy to implement, but the measurement accuracy deteriorates for samples with non-average modulator levels
Solution Approach 1:
The patent divides the calibration process into multiple segments by using two or more calibration solutions with different enzyme modulator concentrations instead of a single solution. This segmentation allows the system to capture the non-linear effects of enzyme modulation across different concentration ranges, thereby improving measurement accuracy for samples with varying modulator levels while maintaining a relatively simple multi-step calibration procedure
Solution Approach 2:
The patent changes the parameter of enzyme modulator concentration in the calibration solutions to create a calibration curve that accounts for modulation effects. By preparing calibration solutions with systematically varied modulator concentrations and measuring the sensor response at each level, the system establishes a relationship between modulator concentration and sensor sensitivity, enabling accurate correction for samples with unknown modulator levels
2Measurement precision
If calibration solutions are prepared to match specific sample modulation levels, then measurement accuracy improves for those specific samples, but the complexity of preparing multiple customized calibration solutions increases
Solution Approach 1:
The patent creates a universal calibration approach where a set of calibration solutions with different enzyme modulator concentrations can be used to calibrate the sensor for any sample regardless of its specific modulator level. The calibration curve generated from these solutions serves multiple purposes: it characterizes the sensor's response across different modulation conditions and provides the basis for correcting measurements of samples with any modulator concentration, eliminating the need for sample-specific calibration
3Measurement precision
If enzyme modulator concentrations in calibration solutions are varied to cover all possible sample conditions, then the measurement accuracy across different samples improves, but the number of calibration solutions and calibration time increase
Solution Approach 1:
The patent applies partial action by selecting a limited number of calibration solutions (two or more) with specifically chosen enzyme modulator concentrations that are sufficient to characterize the sensor's response to modulation. Rather than preparing calibration solutions for every possible modulator concentration, the method uses a strategically selected subset that captures the essential modulation effects, thereby achieving accurate correction without requiring exhaustive calibration across the entire concentration range
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables accurate prediction of enzyme modulation effects in samples, improving the accuracy of creatinine and creatine concentration measurements by accounting for varying enzyme modulation levels, leading to more reliable results compared to existing methods.
Implementation Method 1
Sensors typically use enzymes to convert creatinine and creatine into measurable products, such as hydrogen peroxide which can be detected in an amperometric system
Implementation Method 2
The concentration of Cr (cCr) and the concentration of Crn (cCrn) in an aqueous solution can be determined by amperometric measurement
Data Source
AI summary
A method of calibrating a device for measuring the concentration of creatinine in a sample including one or more enzyme modulators, the method comprising: determining sensitivities of the device for each of two or more calibration solutions, wherein each calibration solution has a different amount of enzyme modulator; determining a degree of modulation for each of the two or more calibration solutions; determining a degree of modulation for a sample to be measured; and calculating the sensitivity of the device for the sample, wherein said calculating comprises modifying the sensitivity of one of the two or more calibration solutions by a function comprising the determined degrees of modulation.