Hydrolases TRIZ Cases
- Inducible Mutagenesis Vectors for Controlled Mutation Rates
Inducible promoters tune mutagenic gene expression to balance genetic diversity with genomic stability during directed evolution.
- Engineered Cas Protein Domain Removal for Genome Targeting
Extracting obstructive Rec1 and Rec2 domains from Cas proteins creates an open architecture that improves polynucleotide accessibility and targeting efficiency.
- Cell Surface Display Homing Endonucleases for Genomic Toxicity Reduction
Cell surface displayed homing endonucleases bypass intracellular expression to eliminate genomic toxicity during high-throughput variant screening.
- Immune Cells with Reduced Androgen Receptor for Prostate Cancer Therapy
Reducing androgen receptor expression in leukocytes overcomes androgen-mediated suppression to improve T cell function and antigen presentation.
- Fanzor Nucleases for Programmable Genome Editing
Fanzor nucleases provide programmable RNA-guided endonuclease activity, expanding genome editing capabilities beyond prokaryotic systems.
- Transformation Plasmid with Endonuclease Sites for Stable Genomic Integration
A transformation plasmid incorporates homologous recombination sequences and endonuclease target sites to deliver genes into host genomes.
- ALAS1 Gene Editing via Modified gRNA for Porphyria Therapy
Chemically modified guide RNAs reduce ALAS1 expression with high cutting efficiency while minimizing off-target effects.
- Lettuce PPO Gene Mutations for Browning Reduction
Targeting distinct PPO genes minimizes cut-surface browning and extends shelf life without external treatments.
- Phi29 DNA Polymerase Mutants Reduce Exonuclease Activity
Site-directed mutations lower exonuclease activity to prevent primer degradation while maintaining high fidelity during DNA amplification.
- Plant Biomass Digestibility via CGR2 CGR3 Enzyme Overexpression
CGR2 and CGR3 enzyme overexpression modifies pectin methylesterification to eliminate harsh chemical pretreatment costs.
- Variant AAV Capsids Enhancing Skeletal Muscle Transduction
Variant AAV capsid polypeptides increase transduction efficiency in human skeletal muscle tissue.
- Acetone Production via Novel Metabolic Pathway
Novel enzymatic pathway converts acetyl-CoA to acetone, uncoupling synthesis from butanol production.
- CRISPR-Cas Lipidation Motif Anchoring for Viral RNA Targeting
Engineered CRISPR proteins use lipidation motifs to anchor to cellular membranes, enabling precise subcellular localization of nuclease activity.
- Yeast Two-Hybrid Vector Library for Deubiquitinating Enzyme Screening
A yeast two-hybrid vector library enables high-throughput screening of deubiquitinating enzyme interactions with target proteins.