Hydrolases TRIZ Cases

Inducible promoters tune mutagenic gene expression to balance genetic diversity with genomic stability during directed evolution.

Extracting obstructive Rec1 and Rec2 domains from Cas proteins creates an open architecture that improves polynucleotide accessibility and targeting efficiency.

Cell surface displayed homing endonucleases bypass intracellular expression to eliminate genomic toxicity during high-throughput variant screening.

Reducing androgen receptor expression in leukocytes overcomes androgen-mediated suppression to improve T cell function and antigen presentation.

Fanzor nucleases provide programmable RNA-guided endonuclease activity, expanding genome editing capabilities beyond prokaryotic systems.

A transformation plasmid incorporates homologous recombination sequences and endonuclease target sites to deliver genes into host genomes.

Chemically modified guide RNAs reduce ALAS1 expression with high cutting efficiency while minimizing off-target effects.

Targeting distinct PPO genes minimizes cut-surface browning and extends shelf life without external treatments.

Site-directed mutations lower exonuclease activity to prevent primer degradation while maintaining high fidelity during DNA amplification.

CGR2 and CGR3 enzyme overexpression modifies pectin methylesterification to eliminate harsh chemical pretreatment costs.

Variant AAV capsid polypeptides increase transduction efficiency in human skeletal muscle tissue.

Novel enzymatic pathway converts acetyl-CoA to acetone, uncoupling synthesis from butanol production.

Engineered CRISPR proteins use lipidation motifs to anchor to cellular membranes, enabling precise subcellular localization of nuclease activity.

A yeast two-hybrid vector library enables high-throughput screening of deubiquitinating enzyme interactions with target proteins.

CRISPR-ExoFISH employs nickase and exonuclease enzymes to denature DNA strands, preserving native chromatin structure while enhancing signal-to-noise ratio.

Heat treatment adjusts sn-1 to sn-2 lysophospholipid ratios, resolving low lecithin content and oxidation issues.

Coating nanoparticles with sequence-specific nucleases overcomes random integration barriers to enable precise genome editing in walled plant cells.

Latent space mapping of single-cell RNA-seq data identifies therapeutic target genes, reducing time consumption and experimental complexity.

Amine protectants prevent formaldehyde from inactivating nitrilase, maintaining high specific activity and productivity.

CRISPR-modified stem cells express PD-L1 and HHLA2 to inhibit NK and T cell rejection, enabling scalable allogeneic therapy.

Modifying the guide RNA 5' end distinguishes it from host cell RNAs, enabling precise targeting of single-stranded nucleic acids.

Acidic enzymatic depolymerization of polyester materials at pH 3 to 6 eliminates salt production and base consumption, reducing industrial processing costs.

A beta-lactamase composition deactivates residual oral antibiotics in the gastrointestinal tract to protect the microbiome.

Replacing customized proteins with programmable RNA guides eliminates manufacturing complexity while maintaining targeting precision.

Segmented nucleic acid scaffolds merge crRNA and tracrRNA components to stabilize Cas9 binding, resolving precision trade-offs in genome editing.

Recombinant E. coli expressing the novel cis-epoxysuccinate hydrolase gene overcomes low wild-type activity to boost L(+)-tartaric acid production efficiency.

CRISPR-Cas12d systems overcome limited homologous recombination rates in plants by introducing targeted double-stranded breaks via nucleic acid-guided cleavage.

Optimizing the Cas9 to gRNA molar ratio resolves low knockout efficiency in terminally differentiated T cells, achieving over 90% TRAC and B2M gene disruption.

Novel RecA enzyme kit resolves detection time and accuracy trade-offs via isothermal amplification, enabling rapid African swine fever virus diagnosis.

Dual strand nickase editing eliminates double-strand breaks and reduces error rates by using reverse transcriptase to synthesize complementary edits.

Engineered glucoamylase variants with targeted amino acid substitutions enhance catalytic activity in fermentation processes.

RNA-guided CRISPR/Cas complexes bind endogenous nucleic acids to enable specific in situ detection without DNA denaturation.

A CRISPR/SpCas9 vector targets a premature termination codon in the wheat Glu-1Ax-null gene to reactivate high-molecular-weight glutenin subunit expression.

Specific amino acid substitutions in lipase variants reduce odor-generating short-chain fatty acid release while maintaining high wash performance.

Circular single-stranded DNA vector protects nucleic acid payloads through duplex formation and controlled nuclease release.

An adapter molecule mediates CPP uptake and releases cargo in endosomes, overcoming entrapment.

Recombineering modifies adenoviral genomes to evade pre-existing immunity while maintaining gene delivery efficacy.

Segmenting CRISPR-Cas9 components for direct injection into fertilized eggs resolves low knock-in efficiency for large gene insertions.

Thermomyces lipase catalyzes asymmetric hydrolysis of N-protected-propargylglycine esters to yield optically active compounds.

Engineered microbes display alpha-galactosidase on their cell surface to hydrolyze raffinose and stachyose, converting waste into fermentable sugars.

Reversible crosslinking and detergent permeabilization isolate antigen-specific plasma cells from mixed populations.

A Dynamin 2 inhibitor downregulates protein expression to restore muscle strength and correct histological features.

Lipid nanoparticles deliver guide RNAs targeting the C5 gene, resolving delivery complexity while reducing hemolysis.

Inhibiting mitofusin shifts cellular metabolism to glycolysis, resolving the contradiction between low reprogramming efficiency and prolonged processing time.

Segmenting complex floral scent pathways into discrete functional modules allows independent control over specific fragrance profiles and plant interactions.

Targeted transposome complexes fragment and enrich specific nucleic acids using unique cellular barcodes, bypassing costly mechanical sorting limitations.

Lipase catalyzes testosterone ester hydrolysis in buffered aqueous media, eliminating caustic soda impurities to achieve pharmaceutical-grade purity.

Cas12i polypeptides with modified PAM recognition enable precise deletions adjacent to motifs while reducing off-target activity.

STI affinity chromatography purifies modified factor Xa derivatives via arginine-based elution, resolving binding characteristic challenges.