Hydrolases TRIZ Cases

Modified Bacillus licheniformis host cells reduce unwanted extracellular proteins to enhance compound purity.

γ-Glutamyltranspeptidase catalyzes the α,γ amide linkage between cysteine and glutamic acid moieties.

An asymmetric CRISPR assay uses competitive crRNA to induce cascade signal amplification.

Chemical modifications to guide RNA alter binding energies and melting temperatures, reducing off-target cleavage while preserving on-target efficacy.

A fluorescent substrate library generates cleavage products for direct protease activity detection in biological samples.

A creatinine sensor calibration method adjusts sensitivity using enzyme modulation factors to maintain measurement accuracy.

MCPP and Perturb-Seq combine CRISPR-Cas9 with single-cell RNA sequencing to map genetic interactions across pooled cell populations.

Gold nanoparticle SERS techniques detect alkaline phosphatase via BCIP intensity ratios, eliminating complex sample pretreatment.

Two-step pH adjustment inactivates proteases in cell culture supernatant, eliminating costly inhibitor removal.

Substituted amino acid residues in a mutant alkaline protease improve solubility and enzymatic activity despite denaturation risks.

Modified pluripotent cells secrete C1-esterase inhibitor protein, reducing angioedema attack frequency and severity.

Point mutations reduce aggregation of mutant Rep proteins, enabling reliable detection of anti-Rep antibodies for multiple sclerosis diagnosis.

Modular TALEN proteins combine TAL DNA-binding domains with FokI nuclease cleavage for precise genome targeting.

Engineered NK cells overexpressing CD16a surface protein.

Downregulating BioF in recombinant hosts redirects carbon flux from biomass growth to pimelyl-ACP intermediates, boosting C7 building block yields.

A modified protein fuses L-asparaginase with a proline and alanine polypeptide to enhance enzymatic activity.

Cas6 endoribonuclease cleaves CRISPR RNA repeats, resolving the contradiction between long transcript recognition and efficient guide RNA generation.

Lyophilized ATP-diphosphohydrolase kits hydrolyze non-microbial ATP to eliminate false positives and accelerate bacterial testing.

A CRISPR Cas9 genome editing system targets specific HSV viral genes to inhibit replication and reduce infection duration.

Recombinant polypeptides express enzymes converting unsaturated fatty acids to lactones, resolving low natural production levels.

Blocking agents mediate nucleic acid coupling to generate a third molecule, resolving reaction control accuracy while managing mixture complexity.

Enzymatic nicking and ligation circularize double-stranded DNA, achieving high yields without bacterial amplification.

Antibodies enable sensitive detection of novel phospholipase B-II proteins, resolving measurement precision challenges in inflammatory disease diagnostics.

Recombinant DNA constructs expressing specific polypeptides increase nitrogen stress tolerance and grain yield under low nitrogen conditions.

Fructose-6-phosphate 3-epimerase shifts reaction equilibrium toward complete conversion, overcoming the 20-35% limit imposed by existing methods.

Mutant-type RNase R tolerates 150 mM NaCl, resolving low salt tolerance and poor expression yield bottlenecks in RNA sample preparation.

A single-base editor composition tracks elapsed time through A-to-G conversion frequency in human genome targets.

A mutant type II beta-lactam acylase with specific amino acid modifications enhances deacylation activity towards adipyl-7-ADCA.

Modifying microRNA expression in CAR-T cells counters tumor microenvironment exhaustion, sustaining anti-tumor activity.

Engineered protease variants prevent amylase inactivation, preserving storage stability and cleaning performance.

Modular CRISPR RNA designs enhance cleavage efficiency and specificity by optimizing distinct functional regions within the nucleic acid construct.

Replacing PSA testing with PDE9A expression measurement resolves low specificity issues by differentiating hormone-resistant and sensitive disease forms.

Serine recombinases mediate stable integration of large DNA sections into specific plant loci without pre-engineering att sites.

MALDI-TOF mass spectrometry detects antibiotic hydrolases using antibody-mediated signal amplification for rapid strain identification.

Digestive protein removes false positive double-stranded nucleic acids with bulges to improve miRNA detection accuracy.

A genetically engineered Bacillus subtilis strain uses CRISPR interference to co-utilize glucose and xylose.

Alpha-dioxygenase converts fatty acids to aliphatic aldehydes via oxidation, eliminating cellular energy burden.

Novel phosphatase enzymes catalyze terpenyl diphosphate cleavage to produce terpene alcohols, resolving the lack of specific biocatalytic pathways.

Cas9-mediated nicking and Direct Label Enzyme labeling enable precise breakpoint detection and copy number estimation.

KAT7 inhibitors alleviate hepatic fibrosis by reducing inflammatory factors.

Cms1 CRISPR systems eliminate pathogenic bacteria via collateral cleavage, bypassing antibiotic resistance mechanisms.

CRISPR-Cas9 editing repairs the CEP290 gene to restore protein function, resolving expression control challenges in LCA10 treatment.

Overexpressing LpxE genes in recombinant bacteria yields monophosphoryl lipid A without acid hydrolysis, simplifying isolation.

Replacing the endogenous F12 locus with a human sequence creates an accurate model for testing reagents.

Targeted amino acid substitutions in thioesterase overcome productivity thresholds, increasing fatty acid yields beyond wild-type limits.

Targeted mutations in the Cas9 REC3 clamp improve mismatch discrimination while maintaining on-target cleavage rates.

Overexpressing IDH, MDH, or GOT enzymes enhances immune cell proliferation and cytotoxicity within nutrient-depleted tumor microenvironments.

Engineered polypeptide catalysts maintain activity and selectivity at 1500 mM substrate levels, overcoming inhibition barriers.

Engineered Corynebacterium strains replace chemical synthesis to boost ornithine and putrescine yields while reducing environmental harm.