Stabilized Protease-Amylase Liquid Detergent
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Prior art protease- and amylase-containing liquid washing agents have inadequate storage stability, leading to a significant loss of enzymatic activity, particularly amylolytic and proteolytic activity, due to the inactivation of amylase by protease, resulting in suboptimal cleaning performance.
Innovation Solution
A liquid washing agent comprising a protease with an amino acid sequence at least 70% identical to a specific sequence (SEQ ID no. 1) and featuring amino acid substitutions R99E or R99D in combination with S3T, V4I, and V199I, used in conjunction with an amylase, which stabilizes the enzymatic activity and maintains improved cleaning performance during storage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If prior art protease and amylase are combined in liquid washing agents, then cleaning performance is improved, but storage stability deteriorates due to protease inactivating amylase
Solution Approach 1:
The invention applies parameter changes by modifying the amino acid sequence of the protease at specific positions (99, 213, 217, 222) to create variant proteases that do not inactivate amylase. These sequence modifications change the protease's properties to eliminate the harmful interaction while preserving its cleaning function, thereby resolving the contradiction between cleaning performance and storage stability.
2Productivity
If liquid washing agents contain protease and amylase, then enzymatic cleaning capability is enhanced, but enzyme activity is lost during storage
Solution Approach 1:
The invention modifies the protease's amino acid parameters at positions 99, 213, 217, and 222 to create stable variants that maintain their activity over extended storage periods. This parameter modification approach allows the washing agent to retain full enzymatic cleaning capability throughout its shelf life, resolving the contradiction between initial productivity and duration of action.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The combination of the modified protease and amylase in the washing agent enhances storage stability, maintaining effective amylolytic and proteolytic cleaning performance after storage, even at low temperatures, and ensures better removal of protease-sensitive soiling on textiles and hard surfaces.
Implementation Method 1
a protease which comprises an amino acid sequence which, over the entire length thereof, is at least 70% identical to the amino acid sequence stated in SEQ ID no. 1
Implementation Method 2
an amylase. Amylases consequently in particular act against starch-containing residues on laundry and catalyze the hydrolysis thereof
Data Source
AI summary
The intention is to improve storage stability in a liquid washing or cleaning agent which contains a protease and amylase. This is achieved by using a protease which comprises an amino acid sequence which, over the entire length thereof, is at least 70% identical to the amino acid sequence stated in SEQ ID no. 1 and, in the numbering according to SEQ ID no. 1, has the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions which are selected from the group consisting of S3T, V4I and V199I.