Hydrolases TRIZ Cases

Split large donor DNA across two AAV vectors to enable CRISPR/Cas9-mediated integration of oversized transgenes.

CRISPR/Cas9 complexes cleave abundant host DNA sequences, reducing sequencing costs by increasing microbial data yield.

A computational method designs stable proteins using structural and ancestral data to generate sequences with multiple amino acid substitutions.

Site-directed mutagenesis enhances lipase thermostability to sustain lipid stain removal in harsh cleaning compositions.

Segmented PROTACs bridge target proteins and the FBXO22 E3 ligase to induce degradation, while CRISPR-Cas9 screens identify compatible ligases.

Dynamin 2 inhibitors resolve non-selective miR-133 agonist toxicity by targeting specific downstream pathways, rescuing muscle phenotypes.

RNA-guided endonucleases introduce targeted double-stranded breaks in embryos to eliminate extra chromosomes.

Fungal deoxyribonuclease disrupts textile biofilms to eliminate persistent malodors and soil redeposition.

Segmented CRISPR-Cas3 delivery overcomes system complexity to achieve precise gene knock-in in poultry cells.

Silencing ZmPLA1E via CRISPR/Cas9 boosts haploid induction rates, reducing stable inbred line development from seven generations to two.

Dual gene fusion creates dsRNA that silences aphid targets, avoiding pesticide resistance and environmental pollution.

Segmenting Cas9 domains and using single-stranded intermediaries minimizes off-target double-stranded breaks to enhance homology-directed repair efficiency.

Demethoxylating natural pectins using pectinmethylesterase under ultra-high pressure yields shelf-stable fruit snacks without added hydrocolloids.

Site-directed mutagenesis of phytase amino acid sequences enhances specific activity and stability, reducing required dosage levels.

BcsG phosphoethanolamine transferase catalyzes in-situ modification of cellulose, resolving low incorporation efficiency and improving digestibility.

Hydrophobic carriers adsorb enzymes to preserve activity, avoiding covalent denaturation and crosslinking agents.

A customized enzyme composition tailored to specific animal needs enhances nutrient digestibility and performance.

NUDIX overexpressing plants scavenge phosphate via enzyme activity, reducing agricultural phosphorous pollution.

Large serine recombinases integrate genetic circuits via orthogonal recognition sites, resolving pre-integration complexity in mammalian genome engineering.

Mutated enzyme maintains catalytic function at 65-75°C, resolving thermal stability limits in oil degumming.

Modifying CasX NTSB and TSL domains expands PAM sequence targeting range while managing system complexity.

A luciferase-HMOX1 cell model enables real-time gene expression tracking for in vitro skin sensitization assays.

A DNase enzyme degrades the extracellular polymeric substance matrix to prevent slime build-up on water-contacted surfaces.

Segmented guide RNA and Cas9 components resolve low precision in gene alteration, reducing ANGPTL3 secretion by five-fold.

Targeted knockout of XylT and FucT genes in Nicotiana eliminates immunogenic beta-1,2-xylosyl and core alpha-1,3-fucosyl residues from produced proteins.

Modified Brassica plants reduce saturated fatty acids to improve nutritional quality and oxidative stability.

Conditional siRNAs activate via biomarkers to inhibit cancer genes, reducing toxicity to healthy cells.

Genetically modified cells metabolize alternative nitrogen, phosphorus, and sulfur compounds to provide selective growth advantage in fermentation processes.

Molecular inhibition of DENND5B expression controls triglyceride absorption to treat hepatic steatosis without unintended metabolic effects.

Marker-assisted selection accelerates soybean variety XR39E14 development by replacing time-consuming phenotypic evaluation with rapid genetic identification.

Specific guide RNA sequences direct Cas9 to cleave JC virus genomes, resolving the contradiction between treatment effectiveness and off-target effects.

Chemical treatment reverses post-mitotic arrest in fused muscle cells, restoring proliferative capacity for tissue regeneration.

CRISPR/Cas9 knockout of the JAK3 gene in mini-pigs creates a severe combined immunodeficiency animal model.

A mutated Shigella apyrase enzyme dephosphorylates organic phosphates with higher affinity through targeted amino acid substitutions.

Packaged phagemids target resistance genes to eradicate specific bacteria without disrupting the intestinal microbiota, preventing dysbiosis.

Enzymatic digestion degrades animal nucleic acids after selective lysis, enabling high-purity microbiome recovery without chaotropic interference.

Segmented CasM domains resolve versatility complexity trade-offs for precise RNA engineering.

Desiccating the biocatalyst suppresses amidase activity to reduce acrylic acid by-products during nitrile conversion.

Translational fusion partners enable efficient yeast secretion of medically valuable proteins requiring correct folding and glycosylation.

Engineered Cas9 scaffolds split into RuvC and HNH domains reduce protein size while maintaining precise genome targeting capabilities.

Replacing hazardous chemical catalysts with biological systems eliminates water-reaction risks while maintaining high production efficiency.

Optimized signal peptides and promoters boost phospholipase D secretion, resolving low yield bottlenecks in enzymatic phosphatidylserine synthesis.

Cas13 polypeptides paired with specific guide RNAs achieve on-target transcript knockdown while limiting off-target activity.

A genetically modified microorganism captures carbon dioxide using RuBisCO and phosphoribulokinase enzymes to synthesize target molecules.

Segmented CRISPR/Cas tau biosensor cells with FRET reporters identify genetic vulnerabilities associated with neurodegenerative disease progression.

CRISPR-Cas9 cleaves the NRAS gene to overcome BRAF inhibitor resistance and prevent tumor relapse.

Pressurized liquid mediates heat exchange in biomass slurry flow processing to prevent localized vaporization and improve cellulose hydrolysis susceptibility.

A FgTad2-FgTad3-Ame1 ternary complex converts adenosine to inosine in mRNA through deamination.

Counter-selectable markers accelerate chromosomal integration in Clostridium thermocellum, overcoming low transformation efficiency bottlenecks.