Hydrolases TRIZ Cases

A haploid inducing line delivers CRISPR-Cas9 machinery via pollen to plant embryos.

PAE protein overexpression modifies storage compound degradation in soybean seeds, raising oil content by 4% and improving crop processing efficiency.

F10 recombinant protein blocks HDAC6 ubiquitin binding, reducing viral replication and inflammation.

CRISPR/Cas9 editing reduces expression of negative regulators, improving drought tolerance and grain yield.

Adding a uridine repeat sequence to the Cpf1 guide RNA increases indel efficiency and reduces off-target activity for precise genome engineering.

Mechanochemical acylation modifies proteins without solvents, eliminating by-products and expanding modification versatility.

A CRISPR-Cas9 mediated gene drive system converts heterozygous genotypes to homozygosity in a single generation.

Targeted insertion at creatine kinase and myoglobin loci resolves epigenetic silencing risks while ensuring long-term therapeutic gene stability.

Host cells expressing modified Candida rugosa lipases selectively cleave ester bonds in fish oil, concentrating EPA and DHA yields.

Site-directed mutagenesis creates stable serine proteases that degrade textile biofilms despite harsh detergent conditions.

Engineered lipase polypeptides catalyze transesterification reactions to produce biodiesel.

Engineered cells in bee bread degrade insecticides via recombinant enzymes.

A recombinant DNA construct uses floral tissue-preferred promoters to drive guided nuclease expression in plant cells.

Mutating the DNA binding cleft of Cas nucleases shifts repair pathways toward homology-driven mechanisms.

Fusing maltose-binding protein to a truncated nuclease prevents host cell toxicity during high-yield intracellular production.

A dual reagent system detects acid phosphatase and alkaline phosphatase markers in urine samples through distinct color changes.

Enzymatic cleavases replace harsh chemical reagents to enable precise, mild peptide sequencing compatible with nucleic acid analysis.

Segmented Ralstonia effectors with localized RVDs enable precise target site prediction without context dependence.

Recombinant arginine deiminase with site-specific fluorescein attachment detects L-arginine via fluorescence intensity changes.

Cooperating cellulases and laccases release sugars from recalcitrant biomass, lowering energy input.

CRISPR/Cas9 editing of 16DOX and GAME4 genes reduces steroidal glycoalkaloids to non-toxic levels while preserving nutritional value for ruminant feed.

Modifying the yvmA gene in Bacillus cells reduces red pigment formation, simplifying protein recovery and purification processes.

Engineered phospholipase D mutants achieve high transphosphatidylation activity to resolve the trade-off between catalytic productivity and enzyme stability.

A multiplexed enzyme assay system determines nine lysosomal activities simultaneously using specific substrates and mass spectrometric analysis.

Constitutive promoters eliminate IPTG induction complexity while maintaining high enzyme levels for efficient L-serine production.

A Cpf1 nickase fused with a cytidine deaminase converts target cytosines to uracils via guide RNA hybridization.

A recombinant host cell expresses a nuclease via the phosphate-responsive pstS promoter to degrade contaminating DNA during fermentation.

A ligation-free method prepares nucleic acid libraries using single-sided PCR and terminal transferase-mediated tailing.

PDE3 modulators induce PDE3A or PDE3B complex formation with SLFN12 to trigger apoptosis in responsive cancer cells.

A mini-DNA synthesizer combines a single reverse transcriptase subunit with a sequence-specific nuclease to enable precise nucleic acid editing.

RecD helicase drives stepwise polynucleotide movement through a nanopore, enabling high salt tolerance and single-base resolution without amplification.

Editing autologous hematopoietic stem cells corrects the genetic defect, resolving graft-versus-host disease risks.

Novel rice promoter sequences drive targeted gene expression to enhance photosynthetic metabolism, increasing seed yield and biomass.

Targeted amino acid substitutions in DGAT1 enzymes boost seed oil content without requiring complex industrial processing methods.

Aptamer complexes internalize CRISPR payloads via cell surface binding.

Meganucleases cleave endogenous immunoglobulin loci to prevent co-production of endogenous antibodies, ensuring purity of humanized transgenic antibodies.

Autologous stem cell correction restores immune function while eliminating graft-versus-host disease risks from allogeneic transplants.

A site-directed integration method generates production-competent Chinese Hamster Ovary cell lines with consistent transcriptional activity.

Mutating ZmYUC2 and ZmYUC4 genes widens brace root growth angles via localized auxin regulation, enhancing lodging resistance without compromising yield.

Replacing ATP with polyphosphate lowers costs while thermophilic enzyme selection maintains activity across 55-80°C.

Measuring clot formation properties optimizes Factor VIII and Factor IX dosages, reducing breakthrough bleeds.

CRISPR editing of the sorghum IAP locus modifies pollen recognition genes to enable cross-compatibility between distinct species.

A genetic control circuit uses a repressor polypeptide to modulate transcription rates in mammalian host cells.

Helitron transposase amplifies DNA copy number using rolling circle replication, overcoming insertion limits of Sleeping Beauty systems.

OMA1 protein expression detects mitochondrial dysfunction in metastatic breast cancer, enabling precise therapy effectiveness assessment.

Isolated urease induces carbonate precipitation in fine sands and silts, avoiding pore plugging from microbial growth while enhancing soil strength.

A Corynebacterium glutamicum strain utilizes a protein variant with a serine-to-phenylalanine substitution at position 109 to enhance L-valine biosynthesis.

CRISPR nucleases target malate dehydrogenase genes to reduce enzyme activity, resolving random integration bottlenecks in plant transformation.