Hydrolases TRIZ Cases

ScFae1 enzyme hydrolyzes bitter kaempferol compounds, reducing levels by 65% to enable safe human consumption.

UndB fatty acid desaturase converts 3-methylcrotonyl-CoA into isobutene through enzymatic decarboxylation.

Genetic disruption of the HPK1 gene via CRISPR-Cas9 knockout overcomes T cell exhaustion markers like PD-1, improving solid tumor infiltration.

Hybrid guide RNAs merge multiple spacers into single transcripts to resolve cassette recombination and enzyme competition during multiplex editing.

Ion-exchange resin removes acrolein impurities from acrylonitrile before enzymatic conversion, preventing catalyst inhibition and ensuring high polymer quality.

Enzymes recognize methylated cytosines and cleave DNA at specific offsets, simplifying methylome generation compared to bisulfite sequencing.

Engineered acyl-ACP thioesterase polypeptides convert pimeloyl-ACP to pimelic acid, replacing petrochemical routes with efficient bio-based production.

Nanoparticle delivery systems transport CRISPR-Cas complexes into eukaryotic cells to enable precise genomic modifications.

CasRx/Cas13d degrades sense and antisense C9orf72 transcripts, reducing toxic proteins across the blood-brain barrier.

CRISPR-Cas9 introduces double-strand breaks at the CEP290 gene to eliminate a cryptic splice site.

Double-strand break induction increases fertile plant recovery frequency by resolving site-specific modification precision trade-offs.

Small molecule inhibitors bind the PAM-interacting domain of Cas9 to resolve the contradiction between high editing efficiency and off-target effects.

Conditionally active promoters in recombinant host cells switch gene expression from propagation to production phases, lowering toxic byproduct accumulation.

Genetically modified Bacillus host cells minimize lipase and esterase production to prevent malodour formation during fermentation processes.

Metagenomic sequencing identifies novel enzymes from uncultivated microbes to resolve scarcity and improve editing specificity.

Mfn modulators restore normal mitochondrial function by regulating autophagy proteins, addressing aberrant cellular processes.

A method constructs guide strand libraries using type II restriction enzymes and methyltransferase activity to prevent further cleavage.

Self-hybridizing guide RNA extensions improve CRISPR delivery efficiency while minimizing toxicity and immunogenicity.

A recombinant microorganism utilizes a cyclic metabolic pathway to assimilate formic acid and carbon dioxide into pyruvate.

Plant chimeric binding polypeptides overcome improper folding and structural stability issues by extracting essential binding functions from immunoglobulins.

Engineered substrate proteins with heterologous cleavage sites trigger hypersensitive immune responses in soybeans upon pathogen protease recognition.

All-In-One Dual CRISPR-Cas12a assay enables rapid visual detection of nucleic acids using a single reaction mixture.

A blood sampling tube containing sodium metavanadate or tungstate salts prevents phospholipase D enzyme activity during collection.

CRISPR-Cas9 genome editing targets specific mutated exons within the dystrophin gene, bypassing delivery limits of the full 2.2 megabase sequence.

Enzyme-catalyzed synthesis produces chiral linkers with high enantiomeric excess, resolving specificity trade-offs in targeted drug delivery.

Homology-independent targeted integration inserts synthetic exons into intronic sequences to enable scalable reporter protein tagging.

Silica nanoparticles with disulfide crosslinks encapsulate biomolecules for plant cell delivery.

Enzymatic conversion of mogroside IIIE yields Compound 1, eliminating bitter metallic aftertastes found in conventional high-intensity sweeteners.

Engineered lipase polypeptides with specific amino acid mutations enhance substrate binding and catalytic activity in cleaning formulations.

Milling semi-crystalline polymers lowers crystallinity, enabling enzymes to degrade plastic waste faster and more efficiently.

Mutant Cpf1 endonucleases introduce single strand breaks to resolve versatility versus specificity trade-offs.

Engineered blocking guide RNAs bind off-target DNA to prevent Cas9 cleavage, resolving the trade-off between editing efficiency and unintended mutations.

Combining mutant and wild-type FATB alleles reduces saturated fatty acids below 7% while maintaining normal plant growth.

Cross-linked polymer coatings resolve the trade-off between scratch resistance and flexibility in touch sensors.

Enzymatic adaptor ligation identifies spatial RNA locations in biological samples using capture probes and sequencing arrays.

An artificial antigen-presenting cell engineered with a multiplex CRISPR-Cas9 system to stimulate T cells.

Controlled selectable marker ratios reduce off-target effects while digital PCR quantifies knock-in efficiency.

Replacing expensive ATP with polyphosphoric acid reduces production costs while class A acid phosphatase maintains high reaction efficiency.

Specific gRNA combinations knock out multiple xenoantigen genes simultaneously, reducing immune rejection in xenotransplantation grafts.

Enzymatic desulfation converts iota-carrageenan to alpha-carrageenan, reducing supply variability and production costs.

Measuring P1GF, PLAP, and sFlt-1 in gingival crevicular fluid enables early detection of gestational diabetes and preeclampsia before adverse effects occur.

A genetically encodable guide RNA recruits endogenous ADAR enzymes to introduce targeted point mutations in selected mRNAs.

A recombinant microbial cell converts xylose into 1,4-butanediol via a six-step shortcut pathway that reduces biosynthetic steps and by-product formation.

CRISPR-mediated genetic constructs arrest undesired trait development in progeny, eliminating post-birth culling and reducing labor costs.

Introducing stem photosynthetic capability via Prunus amygdalus crossing compensates for winter carbohydrate deficiency and boosts almond yield.

Immobilized Candida antarctica lipase B converts ethyl esters to triglycerides, avoiding side products from strong base catalysts and enabling enzyme reuse.

Engineered pNB esterases replace palladium catalysts to remove p-nitrobenzyl groups, increasing yield and selectivity in carbapenem synthesis.