Microorganism Based Processes TRIZ Cases
- Transformation of mycelium into leather, skin, hide and/or textile substitute
See how sequential organic solvent, surfactant, and alkali treatments transform mycelium into l
- Antibiotic-Free Plasmid Production Using Toxin-Antitoxin RNA Control
A toxin-antitoxin RNA selection scheme removes antibiotics from plasmid production, improving purity, safety, yield, and transfection efficiency.
- Engineered Dehydrogenases for Unnatural Cofactor Recycling
Engineered dehydrogenases boost activity with nicotinamide-based unnatural cofactors, improving redox cofactor recycling in biotransformations.
- CLL1 CAR-T Cell Design for Selective AML Targeting
A single-domain antibody CAR targets CLL1+ AML cells to improve tumor killing while reducing the bone marrow toxicity that limits AML CAR-T therapy.
- Modified Microorganism Pathway for High-Yield Aspartic Acid Fermentation
Metabolic edits block competing pathways and relieve phosphoenolpyruvate carboxylase feedback to raise sugar-to-aspartic acid yield.
- Nanoplasmid Replication Origin Switch for Safer High-Yield Vectors
Replacing Pol I origins with a Pol III-dependent origin boosts nanoplasmid yield while avoiding promiscuous replication and antibiotic marker transfer.
- NMN Transporter Variant for Higher L-Glutamic Acid Productivity
A163T substitution in an NMN transporter boosts energy flux in Corynebacterium, increasing L-glutamic acid yield with targeted protein tuning.
- Lignin-Based Drilling Fluid for Filtrate Control and SRB Resistance
Using lignin and biosurfactants, this drilling fluid improves filtrate control and rheology while resisting sulfate reducing bacteria.
- Wine Fermentation Nutrient Composition for Aroma and Browning Control
A yeast-cellulose nutrient blend restores fermentation support after clarification while limiting oxidation, aroma loss, and browning.
- α1,3-Fucosyltransferase Variants for Higher 3-Fucosyllactose Yield
Modified and homologous α1,3-fucosyltransferases raise 3-fucosyllactose productivity by improving enzyme activity in microbial production.
- Ketoreductase Engineering for Stereoselective 1,3-Indane Diol Synthesis
Targeted ketoreductase mutations boost activity and stereoselectivity for making diastereomerically pure 1,3-indane diols used in belzutifan synthesis.
- Partially Hydrolyzed Guar Gum With Low Viscosity and Prebiotic Activity
Controlled β-mannanase hydrolysis lowers guar gum viscosity while preserving prebiotic activity and high solubility for food use.
- Aspergillus Niger Fructosyltransferase for Low-Sucrose FOS Conversion
An Aspergillus niger fructosyltransferase converts sucrose in liquid foods into FOS while limiting hydrolysis, turbidity, and viscosity.
- Lactobacillus Expression Cassette Using PtlpA for High Protein Yield
A PtlpA-based promoter cassette boosts protein expression in Lactobacillus and supports plasmid retention across generations.
- IolT2 Transporter Variant for Higher L-Glutamic Acid Production
Targeted IolT2 amino acid substitution boosts L-glutamic acid productivity in Corynebacterium without broad pathway redesign.
- Mutant Isopropylmalate Synthase for Feedback-Resistant L-Leucine Production
A P247C isopropylmalate synthase mutant reduces L-leucine feedback inhibition and raises enzyme activity for higher-yield production.
- MFS Transporter Variants for Higher Purine Nucleotide Fermentation
Specific amino acid changes in an MFS transporter raise XMP and GMP output in Corynebacterium without changing the overall fermentation process.
- Plasmalogen Synthase Expression for Aerobic Bacterial Production
Introducing an anaerobic plsA gene into aerobic bacteria enables plasmalogen biosynthesis without laborious anaerobic culturing.
- Corynebacterium Protein Activity Tuning for Glutamate Amino Acid Yield
Weakening SEQ ID NO: 1 protein activity in Corynebacterium boosts citrulline and other glutamate-based amino acid yields for fermentation.
- Isopropylmalate Synthase Variant for Feedback-Resistant L-Leucine Yield
A P247 isopropylmalate synthase mutation reduces L-leucine feedback inhibition while preserving catalysis for higher production yield.
- SarA Activation in Actinomycetes for Metabolite Pathway Unlocking
Boosting SarA activity in actinomycetes unlocks biosynthetic pathways, raising metabolite output and revealing 49 new compounds.
- Isopropylmalate Synthase Variant Against L-Leucine Feedback Inhibition
Targeted amino acid substitutions in isopropylmalate synthase overcome L-leucine feedback inhibition and raise fermentation yield.
- L-Homophenylalanine Enzymatic Cascade Using Low-Cost Benzaldehyde
A recombinant E. coli enzyme cascade converts benzaldehyde and pyruvic acid into L-homophenylalanine with high yield and lower cost.
- Beta-Cyclodextrin Production From Sucrose for Higher Purity
An enzymatic sucrose-to-amylose route raises beta-cyclodextrin yield and purity while reducing starch supply risk and byproduct waste.
- L-Valine Producing Strain via α-Acetolactate Flux Redirection
By blocking acetoin and 2,3-butanediol pathways, this engineered strain redirects α-acetolactate flux to raise L-valine yield and purity.
- Thermostable GH16B β-Agarase for High-Purity NA4 and NA6 Production
A thermostable GH16B β-agarase liquefies agarose above its gelling temperature to raise NA4 and NA6 purity and yield.
- Dual-Activity Sialyltransferases for 3′SL and 6′SL Production
A dual α-2,3/α-2,6 sialyltransferase enables one engineered cell to produce 3′SL and 6′SL with lower metabolic burden and stable yield.