MFS Transporter Variants for Higher Purine Nucleotide Fermentation

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Solution Overview

Problem

Existing microorganisms have limited capacity for producing purine nucleotides such as 5'-xanthosine monophosphate (XMP) and 5'-guanosine monophosphate (GMP), necessitating improved production methods to meet increasing demand.

Innovation Solution

Introduction of an MFS transporter variant with specific amino acid substitutions at designated positions in the amino acid sequence, enhancing the purine nucleotide-producing ability of Corynebacterium microorganisms.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If target substance-specific approaches are used to increase purine nucleotide production, then production efficiency is improved, but production capacity remains limited

Engineering Contradiction:

Improvepurine nucleotide production efficiencyVSAvoidpurine nucleotide production capacity

Core Design Contradiction:

ProductivityVSQuantity of substance

Solution Approach 1:

The patent applies parameter changes by modifying amino acid residues at specific positions (185, 238, 259, 283, 310, 354, 378, 383, and 403) of the MFS transporter protein. These parameter changes at the molecular level alter the transporter's function to enhance purine nucleotide production capacity beyond what conventional gene expression modulation can achieve.

Inventive Principle:

Principle #35Parameter changes

2Ease of manufacture

If conventional microorganisms are used for production, then process simplicity is maintained, but production ability is insufficient

Engineering Contradiction:

Improvefermentation process simplicityVSAvoidpurine nucleotide production ability

Core Design Contradiction:

Ease of manufactureVSProductivity

Solution Approach 1:

The patent applies local quality by making specific localized modifications to the MFS transporter protein at defined amino acid positions while leaving the rest of the protein structure and the overall fermentation process unchanged. This allows enhanced production ability while maintaining process simplicity.

Inventive Principle:

Principle #3Local quality

Data Source

PatentEP4700042A1Microorganism producing purine nucleotides and method for producing purine nucleotides by using same

Publication Date: 2026.02.25 CJ CHEILJEDANG CORP

AI summary

Provided are a microorganism producing a purine nucleotide and a method of producing a purine nucleotide using the same.