Recombinant DNA-Technology TRIZ Cases

This case uses stress-conditioned seeds and promoter methylation to improve yield, maturity, and inherited crop stress tolerance.

Nucleosidase treatment converts ribonucleotides into purine bases, overcoming raw material constraints to boost GMP and IMP content in seasonings.

Replacing endogenous exons with human coding sequences at the native locus prevents immune abnormalities while preserving normal T cell populations.

Specific amino acid modifications enable alpha-amylase variants to maintain wash performance and stability against chelating agents and surfactants.

Segmented affinity enrichment with engineered binders resolves binary read-out limitations to map genome-wide oxidation states.

Targeted amino acid substitutions in the IgG4 hinge region reduce host cell protein binding, simplifying purification and lowering manufacturing costs.

Removing the intermediate selection step from Agrobacterium-mediated maize transformation reduces mutation rates while maintaining high efficiency.

Engineered microorganisms produce PHB and PHBH mixtures to accelerate polymer crystallization.

Recombinant hyaluronic acid binding protein eliminates native batch variability and prozone interference to deliver accurate measurement results.

Introducing R-hydratase into Cupriavidus necator converts fatty acid intermediates to monomers, resolving low 3HHx fraction in recombinant strains.

A polypeptide molded article achieves high transparency and strength through hot press molding.

Deoxyketohexose isomerase epimerizes deoxy products at position 3, reducing production steps and improving efficiency in deoxy monosaccharide synthesis.

Combining endoglucanases from GH families 5, 12, and 45 resolves the trade-off between production complexity and enzyme performance by broadening pH stability.

Amino acid aminomutase catalyzes (S)-beta-phenylalanine production while the product precipitates as a solid in the reaction solution.

Decoupling microbial growth from synthesis via oxygen or phosphate control avoids growth inhibition while eliminating expensive inducer costs.

Engineered chymosin variants with specific amino acid substitutions alter proteolytic activity profiles.

Altering DNA gyrase and topoisomerase activity improves L-glutamine productivity by changing the microbial DNA topology.

Sucrose solution expands the perivitelline space in marmoset embryos to facilitate viral vector injection.

ETV2 knockout pigs host human stem cells to generate personalized blood, resolving donor supply limits.

Zebrafish proteins enhance C5-epimerization activity, resolving low efficiency in heparan sulfate production.

Replacing 2,4-D with dicamba in coculture media boosts monocotyledonous transformation efficiency and regeneration rates while reducing genetic mutations.

Mucuna pruriens isoprene synthase resolves low activity bottlenecks in Kudzu and Poplar sources to boost productivity.

AOBGL11p replaces harsh acid hydrolysis by selectively cleaving glucoside bonds under mild conditions, increasing yield and simplifying reaction control.

HG-5epi enzyme epimerizes glucuronic acid residues in N-acetyl heparosan, resolving the bottleneck of limited substrate specificity in known C5-epimerases.

Cellular dedifferentiation triggers promoter demethylation, enabling stable inheritance of stress tolerance and disease resistance across generations.