Mucuna pruriens Isoprene Synthase for High-Activity Production
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Solution Overview
Problem
The existing methods for producing isoprene monomers, particularly using isoprene synthase genes from Kudzu and Poplar, suffer from low enzymatic activity, limiting the productivity of isoprene production.
Innovation Solution
The use of an isoprene synthase derived from Mucuna pruriens, which has a higher specific activity and efficiency in producing isoprene, along with a polynucleotide encoding this enzyme and a transformant system that introduces the isoprene synthase gene into microorganisms like Escherichia coli to enhance isoprene monomer production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If isoprene synthase genes from Kudzu or Poplar are used for fermental production, then isoprene monomer can be produced, but the enzymatic activity is low and productivity is limited
Solution Approach 1:
The patent changes the source parameter of the isoprene synthase enzyme by selecting Mucuna pruriens instead of Kudzu or Poplar. This parameter change results in significantly higher enzymatic activity and productivity, directly resolving the contradiction between reliable enzymatic activity and production productivity.
2Productivity
If isoprene synthase from Mucuna pruriens is used, then enzymatic activity and productivity are significantly improved, but the complexity of the production system increases due to introducing new gene sources and transformant systems
Solution Approach 1:
The patent uses genetic copying by introducing the isoprene synthase gene from Mucuna pruriens into microorganism hosts (such as E. coli or yeast) to create transformants. This copying approach allows the high-activity enzyme to be produced in a controlled, scalable fermentation system, balancing improved productivity with manageable system complexity through standard biotechnological methods.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly increases isoprene monomer production, establishing an efficient and effective isoprene monomer production system with higher productivity compared to previous methods.
Implementation Method 1
an isoprene synthase derived from Mucuna (Mucuna pruriens) is excellent in ability to produce the isoprene monomer
Data Source
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AI summary
The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c): (a) a polynucleotide comprising (i) the nucleotide sequence represented by SEQ ID NO:1, or (ii) the nucleotide sequence consisting of the nucleotide residues at positions 133 to 1785 in the nucleotide sequence represented by SEQ ID NO:1; (b) a polynucleotide that comprises a nucleotide sequence having 90% or more identity to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; or (c) a polynucleotide that hybridizes under a stringent condition with a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of (i) or (ii) above, and encodes a protein having an isoprene synthase activity; and the like.