Transferases TRIZ Cases

Extracellular methylation enzymes modify CpG sites on source DNA to produce controlled methylation levels and patterns.

Engineered polymerase variants control DNA strand movement speed to enable precise single-nucleotide resolution during sequencing.

A cell-free protein synthesis system using isolated plant plastid extracts enables rapid in vitro transcription and translation of genetic designs.

A Kup transporter with a glycine to valine substitution at position 344 increases L-lysine excretion from Corynebacterium glutamicum.

A transposon kit selects plasmid terminal repeats to introduce exogenous genes into host cells.

A double enzyme tandem system converts L-glutamate into L-2-aminobutyric acid using L-glutamate mutase and L-aspartate-beta-decarboxylase.

Concomitant in vitro synthesis of TERT protein and TR RNA subunits enables self-assembly into a functional telomerase complex within eukaryotic lysate.

Selective degradation of low value amino acids by engineered organisms resolves inconsistent composition and toxicity issues in distillers dried grains.

Mutated isopentenyl phosphate kinase bypasses genetic deficiencies in archaea by catalyzing direct phosphorylation of isopentenyl monophosphate.

A heterodimeric benzaldehyde synthase enzyme produces natural benzaldehyde in transgenic plants.

Engineered bacteria convert xylose into mycosporine-like amino acids, overcoming low production efficiency and complex microalgae culturing conditions.

Overexpressing alanine aminotransferase shifts metabolism toward the TCA cycle, resolving low productivity in recombinant protein cultures.

Dicarboxylic acid anhydrides reversibly inactivate thermostable Pol B DNA polymerase to prevent primer degradation and mis-priming during reaction setup.

Protease-deficient extracts and protein chaperones stabilize orthogonal synthetases to produce high yields of biologically active proteins.

Genetic manipulation of Camelina FAD2 and FAE1 genes alters fatty acid biosynthesis pathways to produce specialized oil blends with optimized composition.

DNA synthetic shuffling combines mutations to boost ω-transaminase half-life and inactivation temperature for industrial chiral amine synthesis.

Recombinant DNA methyltransferase produces methylated DNA to enhance bacterial transformation efficiency.

Modified cap analogs with 3'-O-alkyl groups reduce competition with GTP, enabling nearly 100% capping efficiency without post-transcriptional steps.

Enhanced enzyme expression in a transformed microbial cell boosts 2,5-pyridine dicarboxylic acid productivity without requiring additional nitrogen sources.

A peptide prevents ERK1/2 nuclear translocation by blocking importin7 interaction.

Sequential administration of the oligonucleotide inhibitor imetelstat before gemcitabine achieves supraadditive efficacy while reducing systemic toxicity.

A high stringency metabolic selection system using 3,4-dehydroproline and functional P5CS to culture eukaryotic cells.

Recombinant microorganisms convert carbohydrates to long-chain hydrocarbons via engineered anaerobic pathways, eliminating aeration costs and boosting yields.

Engineered error-prone DNA polymerases increase organelle mutation frequencies to generate stable, heritable plant traits like herbicide resistance.

Extracted stilbene synthase and chitinase genes from muscadine grapes improve reliability against Pierce's disease by reducing fungicide dependency.

Modified DNA templates prevent premature transcription termination and reduce dsRNA impurities during in vitro synthesis.

Transaminase catalyzes amino group transfer to produce optically pure chiral amines, overcoming substrate and product inhibition for complete conversion.

Introducing yclM gene copies under strong promoters increases L-lysine production 60-fold, bypassing aspartokinase feedback inhibition limits.

Substituting the ppc gene promoter with cysK enhances L-threonine biosynthesis in recombinant Escherichia coli.

Mutant 3',3'-cGAMP synthases suppress c-di-GMP and c-di-AMP by-products to enable high-purity product isolation.

Engineered Vibrio natriegens strains overcome release factor competition to achieve 25-fold higher yields of non-canonical amino acid proteins.

Mutated microbial strains generate unsaturated alpha,omega-bola glycosides to bypass omega-1 contamination and simplify purification.

Enzymatic intermediaries replace harsh chemicals in selenoneine synthesis, enabling green production of analogs for diverse assays.

A genetically modified cell establishes a fucose salvage pathway to synthesize GDP-fucose from L-fucose.

Deleting beta-oxidation genes prevents intermediate oxidation, allowing high-yield production of medium chain diamines.

Thermococcus archaeal DNA primases enable template-independent nucleic acid synthesis, eliminating primer steps and toxic phosphoramidite solvents.

Eukaryotic hosts express prokaryotic pfa gene clusters to produce DHA and EPA, bypassing slow psychrophilic growth rates.

Heterologous gene clusters convert toxic aldehydes into valuable acids, resolving fermentation inhibition in lignocellulosic biomass processing.

Chimeric recombinant DNA polymerases combine functional domains from multiple thermostable species to synthesize complementary strands with high fidelity.

Overexpressed enzymes in recombinant Clostridium drive metabolic flux through key pathways, resolving low yield limitations in carboxydotrophic fermentation.

Mutant lettuce homoserine kinase alleles increase L-homoserine accumulation to resolve Bremia lactucae resistance without affecting plant growth.

Overexpressing RLK1 reduces soybean rust colonization, mitigating pathogen adaptation and fungicide dependency.

Engineered Escherichia coli strain produces polyhydroxyalkanoate polymers with precise monomer carbon lengths using specific hydratase and synthase enzymes.

Targeted modification of isoprene oligomer terminals via biosynthesis improves wet grip and abrasion resistance without compromising rubber stability.

Recombinant microorganism enhances methionine synthase activity and efflux, resolving purity versus efficiency trade-offs.

A glucosyltransferase enzyme synthesizes poly alpha-1,3-glucan using incompletely refined sucrose substrates.

Targeted mutations in the G and H domains of the beta prime subunit enhance L-lysine production while maintaining strain stability.

Engineered maltooligosyl trehalose synthase mutants boost catalytic activity through targeted amino acid substitutions.