Medium Chain Diamine Production via Recombinant Microorganism Pathway
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Solution Overview
Problem
Current methods for producing medium chain diamines using microorganisms with both ω-oxidative and β-oxidative metabolism pathways result in low yields due to additional oxidation and metabolism issues.
Innovation Solution
A recombinant microorganism is developed by deleting the fatty aldehyde dehydrogenase gene and β-oxidative metabolism pathway-related genes and introducing an ω-transaminase gene, allowing for high-yield production of medium chain diamines by preventing further oxidation and introducing an amine group.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If a microorganism with both ω-oxidative and β-oxidative metabolism pathways is used for medium chain diamine production, then the microorganism can metabolize fatty substrates, but the yield of medium chain diamine is low due to additional oxidation and metabolism of intermediate products
Solution Approach 1:
The patent extracts and removes the harmful metabolic pathways (β-oxidation pathway genes and fatty aldehyde dehydrogenase gene) from the microorganism while retaining the useful ω-oxidation pathway. This is achieved through genetic engineering to delete specific genes responsible for unwanted side reactions that consume intermediate products and reduce diamine yield.
Solution Approach 2:
The patent changes the metabolic parameters of the microorganism by introducing an ω-transaminase gene and deleting specific pathway genes, thereby altering the metabolic flow to favor diamine production. This genetic modification changes the enzymatic parameters that control substrate conversion and intermediate product fate.
2Quantity of substance
If the ω-oxidative metabolism pathway is used to produce medium chain aldehyde intermediates, then the intermediates can be formed, but they undergo further oxidation and β-oxidation reducing the final diamine yield
Solution Approach 1:
The patent converts the harmful effect of intermediate product oxidation into a beneficial process by introducing ω-transaminase that directly converts the ω-aldehyde intermediates into the desired diamine products. The metabolic pathway is redirected so that what would have been wasted through oxidation is now productively converted to diamine.
Solution Approach 2:
The patent changes the metabolic parameter by deleting the fatty aldehyde dehydrogenase gene and β-oxidation pathway genes, preventing further oxidation of intermediates. Simultaneously, the introduction of ω-transaminase changes the enzymatic parameter to favor amine group transfer and diamine formation over oxidation reactions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant microorganism effectively produces medium chain diamines with high yield by optimizing the metabolic pathway, enhancing the production efficiency of medium chain diamines.
Implementation Method 1
an ω-transaminase gene is also introduced
Implementation Method 2
ω-oxidative metabolism pathway
Data Source
AI summary
The present invention relates to a method for producing a medium chain diamine and, more specifically, to a method for producing a medium chain diamine from an alcohol or alkane derived from a fatty acid, by culturing a recombinant microorganism from which a fatty aldehyde dehydrogenase gene in a ω-oxidative metabolic pathway and a β-oxidative metabolic pathway related gene have been deleted, and also into which a ω-transaminase gene has been introduced. The recombinant microorganism disclosed in the present invention can prevent the additional oxidation and β-oxidation metabolism of fatty aldehyde and can produce a medium chain diamine with a high yield by introducing an amine group to the terminus thereof.