ABC-Transporter Microorganisms for Rare-Earth-Assisted Black Dye Production
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Solution Overview
Problem
There is a need to enhance the growth ability and efficiency of microorganisms for producing black dyes, particularly melanin, which is regulated due to its anti-UV properties, and to improve their ability to absorb and utilize rare earth ions to simplify culture conditions and increase yield.
Innovation Solution
A genetically modified microorganism is developed with enhanced rare earth ion absorption capacity by introducing a nucleic acid encoding an ATP-binding cassette transporter (ABC transporter) and tyrosinase, which is cultured in the presence of rare earth elements or ions to improve growth and dye production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional microorganisms are used for black dye production, then culture conditions and parameters require complex setting and optimization, but the yield of target chemicals is limited
Solution Approach 1:
The invention changes the biological parameter of the microorganism by introducing exogenous nucleic acid sequences encoding ABC transporters, which alters the microorganism's ability to uptake rare earth ions. This parameter change enables the microorganism to efficiently utilize rare earth ions as growth-promoting factors, thereby simplifying culture conditions while increasing black dye yield
Solution Approach 2:
The invention introduces ABC transporter proteins as intermediary components that mediate the uptake of rare earth ions into the microorganism. These transporters act as intermediaries between the external rare earth ion source and the microorganism's internal metabolic systems, enabling efficient ion utilization that simplifies culture conditions and enhances productivity
2Productivity
If microorganisms are modified to improve growth ability and enzyme activity through rare earth ions, then yield of target chemicals increases, but the ability to effectively absorb and utilize rare earth ions is insufficient
Solution Approach 1:
The invention modifies the microorganism's physiological parameters by introducing exogenous nucleic acid sequences that encode ABC transporters specialized for rare earth ion uptake. This genetic modification changes the microorganism's ion absorption characteristics, enabling effective utilization of rare earth ions to enhance both growth ability and black dye production
Solution Approach 2:
The introduced ABC transporters enable the microorganism to self-service by autonomously absorbing and utilizing rare earth ions from the culture medium. The microorganism's own metabolic systems are enhanced through this self-acquired capability to uptake essential ions, eliminating the need for complex external optimization
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified microorganism exhibits increased growth rate and improved melanin production capacity, allowing for higher microbial biomass in the same culture time or reduced culture time, and enhanced rare earth ion uptake efficiency.
Implementation Method 1
rare earth ions are related to the growth and enzyme activity of microorganisms... enables microorganisms to effectively absorb and utilize rare earth ions
Implementation Method 2
Biosynthesis of chemicals is mainly achieved by microorganisms that pass raw materials through various enzymatic reactions... nucleic acid encoding tyrosinase
Data Source
AI summary
A genetically modified microorganism producing black dyes is provided. The genetically modified microorganism producing black dyes includes a first exogenous nucleic acid and a second exogenous nucleic acid. The first exogenous nucleic acid includes a nucleic acid encoding an ATP-binding cassette transporter (ABC transporter), wherein the nucleic acid encoding an ATP-binding cassette transporter includes a nucleic acid for ped gene cluster. The second exogenous nucleic acid includes a nucleic acid encoding tyrosinase.