Microorganism Based Processes TRIZ Cases
- Non-Heme Iron Protein Radical Relay for Diverse C(sp3)-H Functionalization
Limited enzymatic C(sp3)-H scope is expanded as iron proteins install azide, chlorine, nitrile, thiocyanate, and trifluoromethyl groups.
- Ferulic Acid Bioconversion to High-Purity Vanillin and 4-Vinylguaiacol
This case uses genome-integrated enzyme pathways to convert ferulic acid into vanillin and 4-vinylguaiacol while limiting by-products.
- Waste-Carbon Biopolymer Films with Fermentation and Blending
This case uses Cupriavidus necator to ferment waste carbon sources, then blends the biopolymer into films meeting ISO and ASTM standards.
- Lactobacillus Plantarum KF511 Composition for Respiratory Inflammation
This case uses Lactobacillus plantarum KF511 to inhibit neutrophil extracellular traps and reduce inflammatory cytokines in asthma and COPD.
- Modified BKT Expression in Microalgae for High-Light Photoprotection
Engineered microalgae use modified BKT to produce astaxanthin, improving high-light photoprotection and biomass productivity.
- Ribulose-Phosphate 3-Epimerase Motif Removal to Reduce Psicose Side Reactivity
Motif II removal in ribulose-phosphate 3-epimerase limits reverse activity and reduces purification needs in psicose production.
- Recombinant Host Cells With TyrH Variants for Higher L-DOPA Yield
Heterologous TyrH variants improve L-tyrosine conversion, raising L-DOPA, dopamine, and S-norcoclaurine production.
- Actinomycete Metabolic Engineering Using Reporter-Guided Single-Cell Sorting
Dual antibiotic-resistance and fluorescent reporters enable high-throughput liquid-culture selection of productive Actinomycete mutants.
- HcCYP87D19-Engineered Yeast for Cucurbitacin Intermediate Production
HcCYP87D19 and HcCYP81Q58 are co-expressed in Saccharomyces cerevisiae to bypass complex plant extraction.
- GPRC5D Bispecific Antibodies for T-Cell Redirection in Myeloma
This case uses GPRC5D/CD3 bispecific antibodies to target myeloma cells, induce ADCC, and redirect T-cell cytotoxicity.
- Genetically Modified Microalgae via Gene Silencing for Food Oils
This case uses CRISPR-Cas gene silencing in microalgae to reshape fatty acids for more suitable food oil production.
- LysE Variant Substitutions for Improved L-Arginine Export
This case uses amino acid substitutions at LysE positions 50, 153, and 215 to favor L-arginine export and raise yield by 20%.
- Engineered RNA Ligases for Scalable Modified Oligonucleotide Synthesis
Chemical synthesis can limit oligonucleotide scale-up; engineered RNA ligases support aqueous enzymatic assembly of modified sequences.
- ABC-Transporter Microorganisms for Rare-Earth-Assisted Black Dye Production
This case uses ABC transporters and tyrosinase to simplify culture conditions while increasing microbial growth and black dye production.