LysE Variant Substitutions for Improved L-Arginine Export

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Solution Overview

Problem

Existing methods for producing L-arginine using microorganisms of the genus Corynebacterium do not adequately address the need for increased production yields, as the lysE gene, which acts as a lysine exporter, also exports L-arginine, leading to suboptimal yields.

Innovation Solution

A LysE variant is developed by substituting specific amino acids at positions 50, 153, and 215 with alternative amino acids to enhance L-arginine export efficiency, thereby increasing production yields.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If the lysE gene is used as a lysine exporter, then lysine export function is provided, but L-arginine production yield is insufficient

Engineering Contradiction:

ImproveL-arginine production yieldVSAvoidexport function specificity

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent applies local quality by making specific amino acid substitutions at positions 50, 153, and 215 of the LysE protein. These localized changes in specific regions of the protein sequence enable the exporter to preferentially transport L-arginine over lysine, thereby improving L-arginine production yield while maintaining export functionality.

Inventive Principle:

Principle #3Local quality

Solution Approach 2:

The patent changes the amino acid sequence parameters of the LysE protein by substituting specific residues at positions 50, 153, and 215. This parameter change in the protein's molecular structure alters its substrate specificity to favor L-arginine export, directly addressing the contradiction between productivity and functional reliability.

Inventive Principle:

Principle #35Parameter changes

2Productivity

If amino acid substitutions are made at positions 50, 153, and 215 of LysE, then L-arginine export efficiency is enhanced, but protein structure complexity increases

Engineering Contradiction:

ImproveL-arginine export efficiencyVSAvoidprotein structure complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The patent makes localized amino acid substitutions at only three specific positions (50, 153, and 215) of the LysE protein rather than altering the entire protein structure. This localized modification approach enhances L-arginine export efficiency while minimizing the increase in overall protein structure complexity.

Inventive Principle:

Principle #3Local quality

Data Source

PatentUS20250230200A1Lyse variant and method for producing l-arginine by using same

Publication Date: 2025.07.17 CJ CHEILJEDANG CORP

AI summary

The present disclosure provides a LysE variant.