Amplification Module Gas Extract Passages Prevent Leakage

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Solution Overview

Problem

Genome extraction devices face issues with reagent leakage due to micro-leakage through layer structures, contamination from vibration-induced perforation of sealing members, and cross-contamination between chambers, leading to inefficient detection processes.

Innovation Solution

A genome extraction device with a dual-chamber structure for reagents, a safety clip to prevent sealing member perforation, and a unique inner chamber design to prevent capillary action, along with a dehumidification unit to maintain bead performance, and separate accommodating portions for primers and probes to prevent mixing of extracts and amplification products.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Device complexity

If a single chamber structure is used for reagents, then device complexity is reduced, but reagent leakage occurs due to micro-leakage through layer structures

Engineering Contradiction:

Improvechamber structureVSAvoidreagent leakage

Core Design Contradiction:

Device complexityVSReliability

Solution Approach 1:

The device is divided into multiple independent chambers (first chamber for reagent, second chamber for bead, third chamber for extract) with separate sealing structures. Each chamber has its own sealing member that independently seals the interface between upper and lower portions, preventing reagent leakage while maintaining manageable device complexity through modular segmentation.

Inventive Principle:

Principle #1Segmentation

2Reliability

If sealing members are used to seal chamber openings, then reagent leakage is prevented, but sealing members are perforated by protrusion members due to vibration during production and distribution

Engineering Contradiction:

Improvesealing effectivenessVSAvoidvibration-induced perforation

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The sealing members are designed with enhanced mechanical strength and structural reinforcement to withstand vibration stresses during production, storage, and distribution before the device is activated. The sealing members are positioned and configured to resist perforation by protrusion members that may form in the cover and outer chamber during handling, ensuring sealing integrity is maintained until the device is ready for use.

Inventive Principle:

Principle #11Beforehand cushioning (Prior cushioning)

3Volume of stationary object

If chambers are positioned close together to save space, then device volume is reduced, but capillary action causes cross-contamination between chambers

Engineering Contradiction:

Improvedevice volumeVSAvoidcross-contamination

Core Design Contradiction:

Volume of stationary objectVSReliability

Solution Approach 1:

Capillary barrier structures are introduced as intermediary elements between the first chamber (reagent) and second chamber (bead). These barriers include hydrophobic porous materials or surface treatments that prevent capillary action from bridging the space between chambers. The barriers allow close positioning of chambers for compact device volume while blocking the capillary forces that would cause reagent to migrate into the bead chamber and cause cross-contamination.

Inventive Principle:

Principle #24Intermediary (Mediator)

4Reliability

If a pad is compressed between upper body and base plate to seal, then sealing is improved, but hole diameter decreases causing incorrect extract capacity to move to amplification module

Engineering Contradiction:

Improvesealing qualityVSAvoidextract capacity

Core Design Contradiction:

ReliabilityVSQuantity of substance

Solution Approach 1:

The sealing function is segmented from the flow control function. Instead of using a single pad structure that performs both sealing and flow regulation, the device uses separate sealing members for sealing purposes and dedicated flow channels with controlled dimensions for extract transfer. This segmentation allows the sealing members to be compressed effectively for reliable sealing without compromising the precise control of extract capacity through the flow channels to the amplification module.

Inventive Principle:

Principle #1Segmentation

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The solution effectively prevents reagent leakage, contamination, and cross-contamination, ensuring efficient genome extraction and amplification processes while maintaining bead performance and allowing for the diagnosis of various diseases through single genome extraction.

Implementation Method 1

a gas moving passage formed on one surface of the body and configured to connect any one of the one or more injection ports to the accommodating portion

Methodology Applied

Scientific EffectGas flow:

Implementation Method 2

an extract moving passage formed on an opposite surface to the one surface of the body and configured to connect the other of the one or more injection ports to the accommodating portion

Methodology Applied

Scientific EffectFluid flow:

Data Source

PatentUS12036545B2Amplification module with gas moving passage and extract moving passage

Publication Date: 2024.07.16 SD BIOSENSOR INC

AI summary

Provided is an amplification module with a gas moving passage and an extract moving passage, more particularly an amplification module in which, when an extract is input from a genome extraction device in which the amplification module is installed, a quantitative amount of the extract is input to each accommodating portion so that the accuracy of detection can be increased.