Aquatic Cell Isolation via Mechanical Sectioning
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for isolating and culturing primary cells from aquatic animal tissues are hindered by the use of chemical reagents or enzymes, leading to short cell lifespan, inconsistency, high costs, and limited production volume, as well as ethical concerns related to animal welfare, particularly for aquatic invertebrates which are sensitive to these reagents.
Innovation Solution
A method involving physical sectioning of aquatic animal tissues without chemical reagents or enzymes, followed by centrifugation and culture with a cell culture medium containing complex antibiotics such as penicillin/streptomycin, amphotericin B, gentamycin, kanamycin, ampicillin, and tetracyclin, to isolate and culture primary cells effectively.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical reagents or enzymes are used for tissue sectioning, then cell isolation efficiency is improved, but cell lifespan decreases and animal welfare issues arise
Solution Approach 1:
The patent replaces chemical reagents and enzymes with a mechanical sectioning device that physically cuts tissues into small pieces. This mechanical approach eliminates chemical exposure to cells, thereby extending cell lifespan while maintaining isolation efficiency through automated high-throughput processing
Solution Approach 2:
The patent introduces a cell culture medium as an intermediary substance that facilitates cell survival and proliferation after mechanical sectioning. The medium compensates for the harshness of mechanical processing and creates a favorable environment for cell growth, thus extending cell lifespan
2Productivity
If chemical reagents or enzymes are used for tissue sectioning, then cell isolation efficiency is improved, but animal welfare issues worsen
Solution Approach 1:
The patent replaces chemical reagents and enzymes with a mechanical sectioning device that physically cuts tissues into small pieces. This mechanical approach eliminates chemical exposure to cells, thereby extending cell lifespan while maintaining isolation efficiency through automated high-throughput processing
Solution Approach 2:
The mechanical sectioning device is designed to automatically process multiple tissue samples in parallel, making the system self-sufficient for high-throughput processing without requiring additional chemical treatments for each sample, thereby improving both efficiency and animal welfare
3Adaptability or versatility
If diverse cell isolation methods are used for different aquatic species, then species-specific requirements are met, but methodology complexity increases
Solution Approach 1:
The patent designs a universal mechanical sectioning device and standardized cell culture medium that can process multiple types of aquatic animal tissues (fish, crustaceans, mollusks) with a single system. This multi-functional approach maintains species-specific adaptability while eliminating the need for multiple different isolation methods, thereby reducing methodology complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enhances the survival and proliferation of aquatic animal cells, reduces microorganism contamination, and addresses ethical concerns by providing a standardized approach for isolating and culturing primary cells from various aquatic species, enabling their use in diverse industries like aquaculture and recombinant protein production.
Implementation Method 1
centrifuging the sectioned tissues to isolate cells from a portion of the sectioned tissues
Data Source
AI summary
The present invention relates to a method for isolation and culture of primary cells from aquatic animal tissues, and specifically, to a method of culturing the tissue of an aquatic animal, comprising physically sectioning it without using a chemical reagent or enzyme and culturing it with a cell culture solution containing complex antibiotics.