Artificial Skin Model Using Coated Cells for Stable Tissue Culture
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Solution Overview
Problem
Existing methods for producing artificial skin models using collagen gel face issues with long-term stability and adhesiveness between layers, requiring special devices and recombinant proteins, which are not practical or efficient.
Innovation Solution
A method involving coated cells with an extracellular matrix component coating film is used to form a dermis tissue layer, allowing for the direct formation of an epidermis layer without collagen gel or special devices, enhancing stability and adhesiveness.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If collagen gel is used as a support for culturing cells, then cell culture can be performed, but long-term stability is degraded and adhesiveness between epidermis layer and dermis layer becomes unstable
Solution Approach 1:
The invention extracts and removes the collagen gel support from the cell culture system. By culturing cells directly on a culture plate without collagen gel, the patent eliminates the source of instability and adhesiveness problems while maintaining cell culture functionality
Solution Approach 2:
The invention introduces a coating film containing extracellular matrix components as an intermediary layer between the culture plate and cells. This coating film provides the necessary cell adhesion and stability without requiring collagen gel, thereby resolving the contradiction between reliability and compositional stability
2Manufacturing precision
If a special device with mesh member and liquid flow-controlling member is used, then high-density dermis-like tissue can be produced, but device complexity increases
Solution Approach 1:
The invention extracts and removes the complex special device (mesh member and liquid flow-controlling member) from the culture system. By using a simple culture plate with coating film, the patent achieves high-density tissue formation without requiring complex device structures
Solution Approach 2:
The invention enables the culture system to self-organize and form high-density tissue structures through the coating film's properties alone, without requiring external mechanical control devices. The cells spontaneously form organized structures on the coating film
3Manufacturing precision
If recombinant protein combining cell growth factor and collagen-bound domain is used, then epidermis layer can be formed, but manufacturing complexity and cost increase
Solution Approach 1:
The invention extracts and removes the complex recombinant protein (combining cell growth factor and collagen-bound domain) from the culture system. By using a simpler coating film containing extracellular matrix components, the patent achieves epidermis layer formation without requiring complex recombinant protein production
Solution Approach 2:
The invention changes the parameters of the coating material from complex recombinant proteins to simpler extracellular matrix components. This parameter change maintains the functionality of epidermis layer formation while significantly simplifying the manufacturing process
Data Source
AI summary
Provided is a novel method capable of producing an artificial skin model. A method for producing an artificial skin model includes: providing coated cells (1), each of which is obtained by covering the surface of a cell (3) with a coating film (2) containing an extracellular matrix component; forming a dermis tissue layer (7), in which the coated cells (1) are laminated, by culturing the coated cells (1); and forming an epidermis layer (12) by arranging epidermis cells (8) on the dermis tissue layer (7).