Tripeptide Mediator for AtaA Polypeptide Separation
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Solution Overview
Problem
The self-aggregation of AtaA polypeptides and their adhesive properties pose challenges in bioprocesses, such as immobilization and biofilm management, where controlling adhesion and separation is crucial but difficult with existing technologies.
Innovation Solution
The use of specific tripeptide sequences like AVL, SVL, or ATL, or their functional equivalents, which can disintegrate self-aggregation of AtaA polypeptides and separate their bonds with other subjects, such as streptavidin, allowing for controlled adhesion and detachment in bioprocesses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If AtaA polypeptides are used for immobilization and adhesion in bioprocesses, then cell self-aggregation and adhesive properties are enhanced, but control over adhesion and separation becomes difficult
Solution Approach 1:
The patent introduces specific tripeptide sequences (AVL, SVL, or ATL) as intermediary substances that mediate between the AtaA polypeptide's strong adhesive property and the need for controlled separation. These peptides act as blocking agents that specifically bind to AtaA, preventing its adhesive function and enabling controlled detachment of immobilized cells or biofilms without requiring harsh conditions.
Solution Approach 2:
The patent changes the chemical parameter by introducing specific amino acid sequences (AVL, SVL, ATL) that have high affinity for AtaA. By adding these peptides with specific chemical compositions, the adhesive state of AtaA can be switched from bound to detached, allowing dynamic control over the adhesion process through simple addition of the peptide solution.
2Productivity
If AtaA polypeptides exhibit high cell self-aggregation property, then immobilization efficiency is improved, but separation and recovery of cells becomes difficult
Solution Approach 1:
The tripeptide sequences serve as intermediary agents that specifically recognize and bind to AtaA polypeptides on cell surfaces. By adding these peptides to the system, aggregated cells can be selectively detached from carriers or surfaces, enabling easy separation and recovery of immobilized cells without damaging the cells or requiring complex separation procedures.
Solution Approach 2:
The patent extracts or removes the adhesive function of AtaA by introducing the tripeptide blocking agents. These peptides specifically bind to AtaA, effectively taking out its adhesive capability and allowing cells to be separated from surfaces or carriers while maintaining cell viability and functionality for reuse.
Data Source
AI summary
The present invention provides a peptide that disrupts the self-aggregation of an AtaA polypeptide or separates a bond between the AtaA polypeptide and another subject. A peptide found by the present inventors has the properties of disrupting the self-aggregation of an AtaA polypeptide or separating a bond between the AtaA polypeptide and another subject. A peptide according to the present disclosure includes amino acid sequences AVL, SVL, ATL, or functional equivalent sequences thereof. In one embodiment, a peptide according to the present disclosure has the ability to separate a bond between an AtaA and other molecules (for example, streptavidin and neutral avidin).