Peptide Preparation Methods TRIZ Cases
- Solid-Phase Peptide Condensation with Carbodiimide Additives
A carbodiimide-based condensing agent and additive improve solid-phase peptide conversion while preventing crystal precipitation.
- Site-Selective Protein Modification Using Lysine-Histidine Acylation Tags
A lysine-histidine acylation tag enables selective protein labeling without metal ions.
- Buckwheat Fractionation for Solvent-Free Protein, Starch, and Fiber Recovery
The process uses aqueous suspension and density separation to recover protein, non-gelatinized starch, and fiber without organic solvents.
- Manufacturing process for the production of linaclotide
This case combines liquid-phase synthesis, RP purification, lyophilization, and ion-pairing chromatography to control trace impurities.
- Proline Endoprotease Gliadin Modification for Stable Water Dispersion
Gradient-pH enzymatic hydrolysis modifies gliadin for uniform dispersion after heat sterilization, salt exposure, and storage.
- Continuous Flow-Through Purification Without Protein A Chromatography
A continuous flow-through sequence replaces Protein A chromatography with chelation, diafiltration, and orthogonal membrane adsorbers.
- Cucurbituril Ternary Complexes for Native Biomolecule Stabilization
A non-covalent cucurbituril host holds two aryl guests, stabilizing biomolecules while enabling rapid release and simple filtration.
- Visible-Light Cysteine Protein Modification for Stable Carbon–Carbon Bonds
Visible-light LADA replaces unstable carbon-sulfur links with stable carbon-carbon bonds while preserving protein chirality.
- Antibody Purification Chromatography for HMW Aggregate and Variant Removal
Affinity, cation exchange, and mixed-mode chromatography reduce HMW aggregates by up to 95% and remove far-basic variants completely.
- Solid-Phase Synthesis of Aromatic-Ring Peptide Derivatives
This case uses a solid-phase carrier, deprotection, condensation, and cyclization to simplify purification across peptide derivatives.
- Target Protein Production in Plants Using Dual-Cassette Agrobacterium Vector
This case combines MacT, RD29B, and a 5'-UTR enhancer with p38 suppression in one vector to reduce culture time and cost.
- Protein and Peptide N-Terminal Modification via Selective Amide Bonding
A carboxylic acid derivative enables simple N-terminal amide bonding in natural proteins and peptides while reducing lysine side reactions.
- Sulfite Capture of Dibenzofulvene During Fmoc Deprotection
Sulfite or bisulfite ions form a stable adduct that prevents dibenzofulvene regeneration and side reactions in peptide synthesis.
- Aqueous Peptide Synthesis with Water-Soluble Condensing Agents
A mixed dispersion of protected amino acids, base, condensing agent, and water enables simpler peptide-bond formation with high purity.