Proline Endoprotease Gliadin Modification for Stable Water Dispersion
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Gliadin's poor dispersibility in water and tendency to aggregate or precipitate under thermal sterilization, long-term storage, or in the presence of sodium chloride limits its applications, and existing methods are complex, use toxic chemicals, or are costly and difficult to control.
Innovation Solution
A method using proline endoprotease to modify gliadin by adjusting the pH value of the solution in a gradient during enzymatic hydrolysis, achieving uniform dispersion in water and maintaining stability under high-temperature sterilization and sodium chloride conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If gliadin is compounded with high-molecular materials to improve dispersibility, then the aggregation and precipitation are avoided, but the operation process becomes complex and the requirements for compounding conditions become extremely high
Solution Approach 1:
The invention extracts and removes the problematic high-molecular material compounding step entirely. Instead of adding external hydrophilic materials, the method uses enzymatic hydrolysis to modify the gliadin itself, extracting the need for complex compounding processes while maintaining the dispersibility improvement.
Solution Approach 2:
The gliadin modifies itself through enzymatic hydrolysis rather than requiring external high-molecular materials. The proline endoprotease catalyzes the hydrolysis of gliadin to produce modified gliadin with improved water dispersibility, making the system self-sufficient without needing additional compounding agents.
2Stability of the object's composition
If chemical reagents are used to treat gliadin to improve dispersibility, then the dispersibility is improved, but toxic chemicals are introduced and product safety is reduced
Solution Approach 1:
The invention converts the potentially harmful effect of chemical modification into a beneficial enzymatic process. Instead of using toxic chemical reagents, the method employs proline endoprotease to catalyze hydrolysis, transforming a harmful chemical approach into a safe biological approach that maintains dispersibility improvement without introducing toxicity.
Solution Approach 2:
The proline endoprotease acts as a safe intermediary catalyst between water and gliadin, enabling hydrolysis without requiring toxic chemical reagents. The enzyme mediates the reaction to produce modified gliadin with improved dispersibility while maintaining product safety.
3Stability of the object's composition
If low-temperature plasma process is used to treat gliadin, then the stability is improved, but the cost is relatively high and the process conditions are not easy to control
Solution Approach 1:
The invention replaces the complex low-temperature plasma process with a simpler enzymatic hydrolysis system. Instead of using plasma technology with difficult-to-control conditions, the method uses proline endoprotease in aqueous solution, substituting a complex physical process with a controllable biochemical reaction.
Solution Approach 2:
The invention changes the reaction parameters from extreme plasma conditions to mild enzymatic conditions. The enzymatic hydrolysis occurs at physiological temperatures and pH values, making the process parameters easier to control and more suitable for industrial production compared to plasma treatment.
4Stability of the object's composition
If compounding with high-molecular materials is used to improve dispersibility, then the aggregation is avoided, but a large number of other components are introduced that significantly weaken the functions of the gliadin particles
Solution Approach 1:
The invention extracts and eliminates the unnecessary high-molecular material components from the system. By using enzymatic hydrolysis to modify gliadin directly, the method removes the need for additional compounding agents that would otherwise introduce extra components and weaken the original gliadin particle functions.
Solution Approach 2:
The invention applies local modification to the gliadin structure through targeted enzymatic hydrolysis. The proline endoprotease specifically hydrolyzes peptide bonds in gliadin to introduce hydrophilic groups at specific locations, improving dispersibility without altering the overall structure and function of the gliadin particles.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified gliadin maintains uniform dispersion and stability, demonstrating improved thermal and salt resistance, and storage stability without the use of high-molecular materials or toxic chemicals.
Implementation Method 1
modifies the gliadin by gradient-adjusting the pH value of solution in the process of an enzymatic hydrolysis reaction
Implementation Method 2
uses a specific proline endoprotease as a modifying agent, and modifies the gliadin by gradient-adjusting the pH value of solution in the process of an enzymatic hydrolysis reaction
Data Source
AI summary
A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.