ATP Regeneration Reaction pH Control Without Buffer Overload
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Solution Overview
Problem
Existing methods for producing substances via enzymatic reactions using ATP coupled with ATP regeneration face challenges in maintaining pH without relying on large amounts of buffer components, leading to increased productivity, raw material costs, and purification load.
Innovation Solution
The method involves carrying out ATP regeneration and enzymatic reactions in the presence of a basic compound containing a divalent metal to maintain pH, thereby improving reaction yield, accumulation concentration, and productivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If buffer components are used to maintain pH in enzymatic reactions with ATP regeneration, then pH stability is improved, but productivity decreases and purification load increases
Solution Approach 1:
The patent removes buffer components from the reaction system and replaces them with a basic compound containing a divalent metal. This extraction of the buffering function from traditional buffer systems allows the reaction to proceed without the drawbacks of buffer usage, thereby improving productivity while maintaining pH stability through the basic compound's pH-regulating properties
Solution Approach 2:
The patent changes the pH maintenance mechanism from buffer-based chemical equilibrium to a system using a basic compound with divalent metal ions. This parameter change in the pH control approach eliminates the need for large amounts of buffer components, reducing purification load and improving overall process efficiency while maintaining stable pH conditions
2Stability of the object's composition
If buffer components are used to maintain pH in enzymatic reactions with ATP regeneration, then pH stability is improved, but purification load increases
Solution Approach 1:
The patent extracts the buffering function from traditional buffer systems and implements it through a basic compound containing a divalent metal. This removal of buffer components from the system simplifies the reaction mixture, reducing purification load and eliminating the complexity associated with buffer removal and purification steps
3Stability of the object's composition
If large amounts of buffer components are used to maintain pH, then pH stability is improved, but raw material costs increase
Solution Approach 1:
The patent removes the need for large amounts of buffer components by implementing pH maintenance through a basic compound with divalent metal. This extraction of the buffering requirement reduces raw material consumption and associated costs while maintaining effective pH control throughout the reaction process
Solution Approach 2:
The patent changes the approach to pH maintenance from using excessive buffer components to using a basic compound with divalent metal ions. This parameter change optimizes material usage, reducing raw material costs while maintaining stable pH conditions necessary for enzymatic reaction efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the simple and efficient production of substances by maintaining pH using a basic compound with divalent metals, enhancing reaction efficiency and reducing costs associated with buffer usage.
Implementation Method 1
carrying out the ATP regeneration reaction and the enzymatic reaction in the presence of a basic compound containing a divalent metal
Implementation Method 2
ATP binds to a divalent metal ion such as an Mg ion (Mg2+) to form a stable complex
Implementation Method 3
polyphosphate kinase in which polyphosphate is a phosphate group donor... capable of regenerating ATP from AMP and ADP by a single enzyme
Implementation Method 4
ATP is used in a variety of enzymatic reactions as an energy source... regenerating ATP from adenosine 5'-diphosphate (ADP) or adenosine 5'-monophosphate (AMP) generated by hydrolysis of ATP
Data Source
AI summary
The present disclosure relates to a method for producing a substance by an enzymatic reaction using ATP.