ATP Regeneration Reaction pH Control Using Divalent Metal Bases
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Solution Overview
Problem
Existing methods for producing substances through enzymatic reactions using ATP coupled with ATP regeneration reactions face challenges in maintaining pH, leading to increased productivity costs and purification loads due to the use of large amounts of buffer components.
Innovation Solution
The method involves maintaining the pH of the ATP regeneration and enzymatic reactions using a basic compound containing a divalent metal, such as magnesium hydroxide or magnesium oxide, to optimize reaction conditions and improve yield and productivity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a buffer solution is used to maintain pH in ATP regeneration and enzymatic reactions, then the pH is maintained within the optimum range, but the amount of buffer component required increases, leading to increased purification load and cost
Solution Approach 1:
The invention changes the pH maintenance approach from using buffer solutions to using a basic compound containing a divalent metal (such as magnesium hydroxide or magnesium oxide). This parameter change allows pH control without requiring large amounts of buffer components, thereby reducing purification load and cost while maintaining reliable pH control within the optimum range for enzymatic reactions
Solution Approach 2:
The invention employs inexpensive basic compounds containing divalent metals (e.g., magnesium hydroxide, magnesium oxide) as alternatives to expensive buffer solutions. These basic compounds can be added in small amounts and effectively maintain pH without requiring the large concentrations of buffer components that would otherwise be needed, thus reducing both cost and purification burden
2Productivity
If an ATP regeneration reaction is coupled with an enzymatic reaction using ATP, then the economic burden of ATP cost is reduced, but the pH changes due to phosphate group exchange and by-product generation, requiring additional pH maintenance measures
Solution Approach 1:
The invention introduces a basic compound containing a divalent metal as an intermediary substance that mediates pH control in the coupled ATP regeneration and enzymatic reaction system. This intermediary absorbs the pH changes caused by phosphate group exchange and by-product generation, allowing the ATP regeneration reaction to proceed efficiently while maintaining pH stability without requiring large amounts of buffer components
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the efficient and simple production of substances by enzymatic reactions, enhancing reaction yield and accumulation concentration without relying on excessive buffer components.
Implementation Method 1
maintaining the pH of the reaction solution by adding a basic compound containing a divalent metal
Implementation Method 2
carrying out an ATP regeneration reaction and an enzymatic reaction using ATP
Data Source
Figure 1A~1B
Figure 1C~2A
Figure 2B~3
AI summary
An object of the present invention is to provide a method for producing a substance, which makes it possible to produce a substance in a simple and efficient manner by an enzymatic reaction using ATP, in which an ATP regeneration reaction is coupled. The method for producing a substance according to an example of the embodiment is a method for producing a substance by an enzymatic reaction using ATP, in which the enzymatic reaction and an ATP regeneration reaction of reacting ADP or AMP with a phosphate donor to generate ATP are carried out in one reaction solution in the presence of a basic compound containing a divalent metal.