Avatar Mouse Model for Atopic Dermatitis Immune Replication

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Solution Overview

Problem

Current animal models do not effectively represent the specific immune response of atopic dermatitis patients, particularly those with house dust mite-induced conditions, limiting the development of tailored therapeutic or preventive agents.

Innovation Solution

An avatar mouse model is created by injecting peripheral blood mononuclear cells (PBMCs) from atopic dermatitis patients into immunodeficient mice, specifically an NSG mouse, to replicate the immune response and skin characteristics of atopic dermatitis caused by house dust mites, allowing for the screening of therapeutic or cosmetic agents.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional animal models are used, then general immune response can be examined, but specific immune response of atopic dermatitis patients cannot be represented

Engineering Contradiction:

Improveaccuracy of immune response representationVSAvoidcomplexity of animal model generation

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent creates a copy of the patient's immune system by transplanting human PBMCs into immunodeficient mice, generating avatar mice that replicate the specific immune response characteristics of atopic dermatitis patients. This copying approach allows accurate representation of patient-specific immune responses while using a standardized mouse model platform.

Inventive Principle:

Principle #26Copying

Solution Approach 2:

The patent uses immunodeficient mice as an intermediary host to carry human PBMCs, enabling the study of human immune responses in an animal model. The mice serve as a mediator that allows human immune cells to function in vivo while providing a controllable animal model system.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Adaptability or versatility

If patient-specific immune response is accurately represented, then tailored treatment can be developed, but model generation becomes more complex

Engineering Contradiction:

Improveapplicability to patient-specific treatmentVSAvoidcomplexity of model generation process

Core Design Contradiction:

Adaptability or versatilityVSDevice complexity

Solution Approach 1:

By copying individual patient's PBMCs into avatar mice, the patent enables patient-specific treatment development while maintaining a standardized model generation protocol. Each patient's immune characteristics are replicated in their own avatar mouse, allowing personalized treatment screening without requiring entirely new model systems.

Inventive Principle:

Principle #26Copying

Solution Approach 2:

The patent segments the immune system study into distinct components by using PBMCs that can be isolated, cultured, and transplanted separately. This segmentation allows flexible combination with different immunodeficient mouse strains and treatment protocols, enhancing adaptability while maintaining procedural standardization.

Inventive Principle:

Principle #1Segmentation

3Measurement precision

If human PBMCs are transplanted into immunodeficient mice, then patient-specific immune response is achieved, but differentiation from mouse immune cells becomes difficult

Engineering Contradiction:

Improveaccuracy of immune response measurementVSAvoiddifficulty in distinguishing human and mouse cells

Core Design Contradiction:

Measurement precisionVSDifficulty of detecting and measuring

Solution Approach 1:

The patent employs fluorescent labeling and immunohistochemical staining to visually distinguish human PBMCs from mouse immune cells in avatar mice. These detection methods enable precise identification and measurement of human immune cell responses without confusion from mouse endogenous cells.

Inventive Principle:

Principle #32Color changes

4Reliability

If current mouse models are used, then general skin immune response can be studied, but atopic dermatitis-specific characteristics are missed

Engineering Contradiction:

Improverelevance to atopic dermatitis pathologyVSAvoidease of model generation

Core Design Contradiction:

ReliabilityVSEase of manufacture

Solution Approach 1:

The patent copies the specific immune pathology of atopic dermatitis patients into avatar mice by transplanting their PBMCs. This approach reliably reproduces atopic dermatitis-specific immune characteristics including Th2-dominated responses and allergen-specific reactions, while using the well-established immunodeficient mouse platform that maintains ease of model generation.

Inventive Principle:

Principle #26Copying

Data Source

PatentUS20230337642A1Method for producing avatar mouse as atopic dermatitis animal model and use thereof

Publication Date: 2023.10.26 IND ACADEMIC COOP FOUND YONSEI UNIV

AI summary

The present invention relates to a method for producing an avatar mouse, which is an animal model of atopic dermatitis, and a method of screening a therapeutic agent for atopic dermatitis using the same. According to the present invention, it is possible to more effectively identify a patient-specific immune response by representing an immune response in an actual atopic dermatitis patient. Thus, the present invention is expected to be widely used in the development of a new immunotherapy system enabling tailored treatment.