Bacillus mojavensis H12 Microbial Conversion of High-Molecular-Weight Pullulan
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Solution Overview
Problem
Current methods for producing low-molecular-weight pullulan rely on Aureobasidium pullulans and use glucose or sucrose as substrates, lacking a microbial conversion method for high-molecular-weight pullulan.
Innovation Solution
Utilization of Bacillus mojavensis H12, which produces pullulanase, to convert commercial high-molecular-weight pullulan into low-molecular-weight pullulan through a microbial conversion process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If Aureobasidium pullulans is used for fermentation production of low-molecular-weight pullulan with glucose or sucrose as substrate, then pullulan can be produced, but there is no microbial conversion method for high-molecular-weight pullulan
Solution Approach 1:
The patent changes the substrate parameter from glucose/sucrose to high-molecular-weight pullulan, and changes the producing organism parameter from Aureobasidium pullulans to Bacillus mojavensis H12, which naturally produces pullulanase enzyme. This parameter change enables microbial conversion of high-molecular-weight pullulan into low-molecular-weight pullulan, expanding substrate utilization capability.
2Manufacturing precision
If commercial high-molecular-weight pullulan is converted into low-molecular-weight pullulan, then molecular weight is reduced for better diffusivity, but the conversion process requires specific enzymatic conditions
Solution Approach 1:
The Bacillus mojavensis H12 strain naturally produces pullulanase enzyme itself, eliminating the need to add external enzymes or complex conversion systems. The strain performs the molecular weight reduction function autonomously through its endogenous enzymatic activity during fermentation, simplifying the overall process while achieving precise molecular weight control.
Solution Approach 2:
The patent optimizes fermentation parameters including temperature (28-37°C), pH (6.0-7.0), and cultivation time to control the enzymatic conversion process. By adjusting these parameters, the molecular weight of pullulan can be precisely controlled to meet different application requirements.
3Productivity
If pullulanase producing property of Bacillus mojavensis H12 is utilized, then conversion efficiency is improved, but fermentation conditions must be optimized
Solution Approach 1:
The patent optimizes fermentation parameters including initial pH (6.0-7.0), temperature (28-37°C), inoculum size (1-5%), and cultivation time (24-72 hours) to maximize pullulanase production and conversion efficiency. These parameter optimizations enable high productivity while maintaining process simplicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Achieves the conversion of high-molecular-weight pullulan to low-molecular-weight pullulan with controlled molecular weight and low production costs, suitable for various applications in foods and cosmetics.
Implementation Method 1
the application of Bacillus mojavensis H12 in producing a pullulanase by fermentation
Implementation Method 2
utilizing the pullulanase producing property of Bacillus mojavensis H12 to convert commercial high-molecular-weight pullulan into low-molecular-weight pullulan
Data Source
AI summary
A Bacillus mojavensis H12 is disclosed, wherein the Bacillus mojavensis H12 was deposited in China General Microbiological Culture Collection Center on Apr. 23, 2023, with a deposit number of CGMCC No. 27186 and a classification name of Bacillus mojavensis, and a 16S rDNA sequence is shown in SEQ ID No. 1; a commercial high-molecular-weight pullulan is converted into a low-molecular-weight pullulan by utilizing the pullulanase producing property of Bacillus mojavensis H12, which provides a basis for the industrial production of the low-molecular-weight pullulan.