Bacillus proteolyticus CWJ-2-GJ Irrigation for Acanthopanax Leaf Yield
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Solution Overview
Problem
The scarcity of Acanthopanax senticosus resources and the need for efficient cultivation methods to enhance the content of medicinal active substances in its leaves, particularly due to the limited availability of roots and stems, necessitates the development of microbial agents that can promote the growth and yield of medicinal ingredients in the leaves.
Innovation Solution
The isolation and application of Bacillus proteolyticus CWJ-2-GJ, deposited as CGMCC No. 29159, which is used to irrigate potted seedlings of Acanthopanax senticosus, enhancing the biomass and yield of medicinal ingredients such as Eleutheroside B, chlorogenic acid, and hyperoside in the leaves.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical fertilizers and pesticides are used to promote plant growth, then productivity and yield are improved, but environmental pollution and loss of substance increase
Solution Approach 1:
The patent changes the chemical composition parameters of the fertilizer by using organic materials (chicken manure, rice bran, soybean powder) instead of synthetic chemicals, transforming the substance type from inorganic to organic while maintaining nutrient content for plant growth
Solution Approach 2:
The patent converts potential harmful substances into beneficial ones by using organic waste materials (chicken manure, rice bran) that would otherwise be pollutants, transforming them into nutrient-rich fertilizers that promote plant growth without environmental harm
2Quantity of substance
If wild Acanthopanax senticosus resources are harvested, then medicinal material supply is improved, but resource scarcity and loss of substance worsen
Solution Approach 1:
The patent applies preliminary action by treating the plants with organic fertilizer before harvest to enhance the content of medicinal active substances in the leaves, thereby increasing the medicinal value and quantity of usable material from the same plant resources
Solution Approach 2:
The patent shifts from harvesting rare root and stem resources to cultivating and harvesting leaf resources, creating a substitute product (leaves with enhanced medicinal compounds) that copies the therapeutic function while being more abundant and renewable
3Productivity
If conventional cultivation methods are used, then operation simplicity is maintained, but productivity and medicinal ingredient yield remain low
Solution Approach 1:
The patent applies preliminary action by preparing and applying organic fertilizer to the plants before the growing season begins, allowing the nutrients to be absorbed and accumulated during the growth period, thereby enhancing productivity without adding complexity to the main cultivation process
Solution Approach 2:
The patent employs self-service by using natural organic materials (chicken manure, rice bran, soybean powder) that decompose and release nutrients automatically in the soil, eliminating the need for complex fertilization systems or mechanical intervention while maintaining simple cultivation operations
Data Source
AI summary
A Bacillus proteolyticus CWJ-2-GJ and an application thereof are provided. The deposit number of the Bacillus proteolyticus CWJ-2-GJ is CGMCC No. 29159, and the Bacillus proteolyticus CWJ-2-GJ is isolated from the rhizosphere soil of Acanthopanax senticosus. The potted seedlings of Acanthopanax senticosus are irrigated with the bacterial liquid of the Bacillus proteolyticus CWJ-2-GJ.