Particle-Attached Bacterial Compositions for Stress-Resistant Colonization

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Solution Overview

Problem

Existing bacterial compositions face challenges in surviving harsh environmental conditions during delivery and achieving successful colonization and expansion at the site of interest, leading to suboptimal therapeutic effects.

Innovation Solution

A composition comprising bacteria partially attached to particles made of polysaccharide-based materials and metal carbonates, which allows for increased bacterial attachment and viability, even under stressful conditions, by using a method that includes contacting bacteria with these particles in a solution and allowing them to attach.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If bacteria are delivered in conventional compositions, then delivery is straightforward, but bacterial survival and colonization under harsh environmental conditions is poor

Engineering Contradiction:

Improvebacterial survival and colonizationVSAvoidharsh environmental conditions

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent introduces particles as an intermediary carrier that mediates between the bacteria and harsh environmental conditions. These particles provide a protective interface that shields bacteria from stressors while enabling delivery to target sites, resolving the contradiction between straightforward delivery and bacterial survival.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent modifies environmental parameters by creating a localized protective microenvironment around bacteria through particle attachment. This changes the physical and chemical parameters (pH, osmotic pressure, temperature resistance) in the immediate vicinity of bacteria, allowing them to survive harsh conditions that would otherwise be lethal.

Inventive Principle:

Principle #35Parameter changes

2Quantity of substance

If bacteria are attached to particles, then bacterial load and retention are increased, but inert material content increases and bacterial viability may be compromised

Engineering Contradiction:

Improvebacterial load and retentionVSAvoidinert material content

Core Design Contradiction:

Quantity of substanceVSLoss of substance

Solution Approach 1:

The patent applies local quality by creating particles with specific localized properties - hydrophobic regions for bacterial attachment and hydrophilic regions for dissolution. This allows different parts of the particle system to serve different functions: providing structural support where needed while enabling dissolution to reduce inert material in other regions.

Inventive Principle:

Principle #3Local quality

Solution Approach 2:

The patent employs discarding and recovering by designing particles that are temporarily present during delivery and attachment but are subsequently discarded (dissolved) after serving their protective function. This reduces inert material content in the final formulation while maintaining bacterial load during the critical delivery phase.

Inventive Principle:

Principle #34Discarding and recovering

3Stability of the object's composition

If particles are made water-insoluble, then bacterial attachment stability is improved, but particle dissolution and reduction of inert material is hindered

Engineering Contradiction:

Improvebacterial attachment stabilityVSAvoidparticle dissolution and inert material reduction

Core Design Contradiction:

Stability of the object's compositionVSEase of manufacture

Solution Approach 1:

The patent applies segmentation by dividing particles into distinct functional regions with different solubility characteristics. Hydrophobic segments provide stable attachment surfaces for bacteria, while hydrophilic segments enable controlled dissolution. This segmentation resolves the contradiction between stability and dissolution capability.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent uses composite materials combining hydrophobic and hydrophilic components within the same particle system. This composite structure allows simultaneous achievement of water-insolubility (for stability) in certain regions and water-solubility (for dissolution) in other regions, resolving the apparent contradiction.

Inventive Principle:

Principle #40Composite materials

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition achieves enhanced bacterial attachment and load, increased resistance to environmental stress, and prolonged retention on mucous surfaces, facilitating successful colonization and therapeutic efficacy.

Implementation Method 1

a population of at least one strain of bacteria at least partially attached to particles

Methodology Applied

Scientific EffectAdhesion: Adhesive

Data Source

PatentUS20250270536A1Bacterial compositions and methods for growing bacteria on particles

Publication Date: 2025.08.28 MYBIOTICS PHARMA LTD

AI summary

The present invention is directed, inter alia, to compositions comprising a population of at least one strain of bacteria at least partially attached to particles, the particles comprise (i) a polysaccharide-based material; and (ii) a metal carbonate. Uses of the compositions, e.g., for prevention or treatment of dysbiosis, and methods for the preparation thereof are further provided herein.