Bakery Enzyme Composition for Short Bite Without Crumbliness Loss

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Solution Overview

Problem

Existing methods to improve the short bite of bakery products, such as the use of unsaturated monoglycerides and thermostable serine or metallo-proteases, face limitations including the need for specific labeling and adverse effects on crumbliness and resiliency, necessitating a more effective solution.

Innovation Solution

A combination of a thermophilic serine protease with an optimum activity above 70°C and a lipase is used in bakery applications, particularly in bread making, to enhance the short bite of bakery products.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of operation

If unsaturated monoglycerides are used to improve short bite, then the bakery product becomes easier to bite, but specific labeling with E-number is required which prevents some consumers from buying the product

Engineering Contradiction:
Improveshort biteVSAvoidlabeling requirement
Core Design Contradiction:
Ease of operationVSObject-generated harmful factors

Solution Approach 1:

The patent changes the chemical parameter by using a specific ratio of monoglycerides (1-5%) combined with proteases instead of higher amounts of monoglycerides alone, achieving the same short bite improvement without requiring excessive emulsifier labeling

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite system combining monoglycerides with proteases (specifically thermophilic proteases like subtilisin Carlsberg or subtilisin BPN') to achieve synergistic effects on short bite, allowing reduced emulsifier content while maintaining functionality

Inventive Principle:
Principle #40Composite materials

2Ease of operation

If high amounts of thermostable serine or metallo-protease are used to improve short bite, then the bakery product becomes easier to bite, but the crumbliness and resiliency of the product deteriorate

Engineering Contradiction:
Improveshort biteVSAvoidcrumbliness and resiliency
Core Design Contradiction:
Ease of operationVSStrength

Solution Approach 1:

The patent optimizes the protease concentration parameter to a specific range (0.01-0.1% based on flour weight) and combines it with monoglycerides, achieving improved short bite while maintaining dough strength and product crumbliness through the synergistic interaction between the two components

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent introduces monoglycerides as an intermediary substance that works in conjunction with proteases to modify protein-protein interactions in the crumb structure, thereby improving short bite without compromising the structural integrity and resiliency provided by the gluten network

Inventive Principle:
Principle #24Intermediary (Mediator)

3Ease of operation

If intermediate thermostable or thermostable serine or metallo-protease is used to improve short bite, then the bakery product becomes easier to bite to a certain extent, but the improvement is limited and high amounts are detrimental to other properties

Engineering Contradiction:
Improveshort biteVSAvoidoverall product quality
Core Design Contradiction:
Ease of operationVSProductivity

Solution Approach 1:

The patent merges the functions of monoglycerides (emulsifiers) and proteases (protein-modifying enzymes) into a single dough treatment system, where the protease component specifically targets protein-protein interactions to improve short bite, while the monoglyceride component provides additional textural benefits and stabilizes the system

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The synergistic effect of this enzyme combination significantly improves the short bite of bakery products, reducing the maximum force needed to break the product by at least 15% compared to using either enzyme alone, without adversely affecting dough rheology or crumb structure.

Implementation Method 1

the use of combination of a thermophilic serine protease having an optimum activity at a temperature higher than 70°C

Methodology Applied
Scientific EffectProteolysis: Hydrolysis

Implementation Method 2

at least one second enzyme, wherein said second enzyme is a triacylglycerol lipase or triacylglycerol acylhydrolase

Methodology Applied
Scientific EffectLipolysis: Hydrolysis

Data Source

PatentEP3481205B1Improved bakery composition
Publication Date: 2026.01.07 PURATOS NV
  • EP3481205B1 patent drawingFigure 1A~1B
  • EP3481205B1 patent drawingFigure 1C
  • EP3481205B1 patent drawing

AI summary

It has been found that the combination of enzymes, in particular the combination of a thermophilic serine protease and a lipase, are able to improve the short bite in bakery products. Provided herein are compositions comprising these enzymes, the use of this combination of enzymes and methods for preparing bakery products using the combination of a thermophilic serine protease and a lipase.