Position-349 BBD29_RS02010 Variant for High-Yield L-Arginine Production
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Solution Overview
Problem
There is a need for improved methods to efficiently produce L-arginine with high yield using microorganisms.
Innovation Solution
A novel BBD29_RS02010 variant polypeptide, where arginine at position 349 is substituted with glutamine, is used in a microorganism culture to enhance L-arginine production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional methods (increasing expression of arginine biosynthesis genes or deleting unnecessary genes) are used, then L-arginine production is improved, but the yield is still insufficient to meet growing industrial needs
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the BBD29_RS02010 enzyme at position 349, substituting arginine with glutamine to create variants with enhanced catalytic activity. This specific parameter change in the enzyme structure results in improved L-arginine production yield that exceeds conventional methods, directly addressing the insufficiency of existing approaches to meet industrial demand
2Productivity
If target material-specific approaches are used (increasing expression of arginine biosynthesis genes), then L-arginine production is enhanced, but the efficiency is limited and further research is needed
Solution Approach 1:
The patent applies local quality by making a specific localized modification to the BBD29_RS02010 enzyme at position 349, where arginine is substituted with glutamine. This localized change in the enzyme's amino acid sequence creates variants with superior catalytic activity for L-arginine production, achieving high efficiency without requiring complex multi-gene manipulation approaches
Data Source
AI summary
Provided are a novel BBD29_RS02010 variant polypeptide; a polynucleotide encoding the variant polypeptide; a microorganism comprising any one or more of the variant polypeptide, the polynucleotide encoding the variant polypeptide, and a vector comprising the polynucleotide; and a method of producing L-arginine, the method comprising the step of culturing the microorganism in a medium.