Beta-1,3-1,6-Glucan Detection via Dual-Binding Complex
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Solution Overview
Problem
Current methods, such as the Limulus reaction and Patent Document 1, cannot distinguish between fungal-derived and plant-derived β-glucan containing β-(1→3) bonds, leading to false positives in deep mycosis tests, especially when plant-derived β-glucan is introduced into the human body during medical procedures.
Innovation Solution
A method involving molecules that specifically bind to β-(1→3) and β-(1→6) bonds, allowing for the detection of β-1,3-1,6-glucan by forming a complex with both binding molecules and measuring the amount of β-1,3-1,6-glucan in a sample, using molecules like horseshoe crab-derived factor G, dectin-1, or β-glucan recognition proteins, and enzyme-inactivated mutants of glucanases and antibodies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the Limulus reaction or conventional β-glucan detection methods are used, then β-glucan detection is achieved, but fungal-derived and plant-derived β-glucan cannot be distinguished
Solution Approach 1:
The detection method is segmented into multiple specific binding steps: first binding to β-(1→3) bonds, then binding to β-(1→6) bonds. This segmentation allows the method to detect not only the presence of β-glucan but also its specific structural composition, thereby distinguishing fungal-derived β-1,3-1,6-glucan from plant-derived β-glucan.
Solution Approach 2:
The invention changes the detection parameters from general β-glucan detection to specific bond-type detection. By using molecules that specifically bind to β-(1→3) bonds and β-(1→6) bonds separately, the method detects specific structural parameters of the glucan, enabling source identification based on the characteristic β-1,3-1,6-linkage structure of fungal glucan.
2Reliability
If conventional detection methods are used, then β-glucan is detected, but false positives occur when plant-derived β-glucan is present
Solution Approach 1:
The invention introduces specific binding molecules as intermediaries that mediate between the β-glucan and the detection system. These intermediaries (molecules specifically binding to β-(1→3) bonds and β-(1→6) bonds) act as selective filters that only allow fungal-derived β-1,3-1,6-glucan to be detected, blocking plant-derived β-glucan from producing false positive signals.
3Device complexity
If single-binding molecule methods are used, then detection is simpler, but specificity to β-1,3-1,6-glucan is reduced
Solution Approach 1:
The invention merges two binding steps into a unified detection system: first binding to β-(1→3) bonds and then binding to β-(1→6) bonds. This merging of multiple specific binding events creates a detection method that is highly specific to β-1,3-1,6-glucan while maintaining operational simplicity through sequential application of the binding molecules.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables accurate quantification of β-1,3-1,6-glucan, preventing false positives and allowing for precise evaluation of fungal infections by distinguishing fungal-derived β-glucan from plant-derived β-glucan, thus improving the reliability of deep mycosis tests.
Implementation Method 1
a step for mixing β-glucan in a test sample, a molecule that specifically binds to a β-(1→3) bond, and a molecule that specifically binds to a β-(1→6) bond to form a complex containing the aforementioned molecule that specifically binds to a β-(1→3) bond and the aforementioned molecule that specifically binds to a β-(1→6) bond
Data Source
AI summary
The present invention provides a method for quantitatively detecting β-1,3-1,6-glucan separately from β-1,3-glucan and β-1,3-1,4-glucan. The present invention is a method for measuring β-1,3-1,6-glucan, the method including: a step for mixing β-glucan in a test sample, a molecule that specifically binds to a β-(1→3) bond, and a molecule that specifically binds to a β-(1→6) bond to form a complex containing the molecule that specifically binds to a β-(1→3) bond and the molecule that specifically binds to a β-(1→6) bond; a step for detecting the complex; and a step for measuring the amount of β-1,3-1,6-glucan in the test sample, on the basis of the results of the detection.