Bifidobacterium Pseudocatenulatum Strain for Xylan Use in Milk Media
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Solution Overview
Problem
Bifidobacterium pseudocatenulatum YIT 11057 has limited viability in milk component-containing media, hindering its effectiveness in fermented milk production.
Innovation Solution
A novel bacterium belonging to Bifidobacterium pseudocatenulatum with a xylanase gene, represented by SEQ ID NO: 1, exhibits enhanced viability in milk component-containing media, maintaining a survival rate of 20% or more after 24 hours and 2 weeks of preservation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If Bifidobacterium pseudocatenulatum YIT 11057 is used as a probiotic candidate, then it can efficiently utilize xylans and produce organic acids, but it does not have sufficient viability in milk component-containing medium
Solution Approach 1:
The patent applies parameter changes by identifying and modifying specific genetic parameters (genes) of Bifidobacterium pseudocatenulatum YIT 11057 to improve its viability in milk component-containing medium while preserving its xylan utilization capability. This involves genomic analysis and strain optimization to adjust biological parameters without losing key functional abilities.
2Productivity
If a bacterium is selected for high xylan utilization ability, then it can actively metabolize carbohydrates in intestines, but it may not maintain sufficient viability in milk-based fermented food production
Solution Approach 1:
The patent applies universality by selecting and optimizing a bacterial strain that can perform multiple functions: utilizing xylans for carbohydrate metabolism in the intestine while simultaneously maintaining viability in milk component-containing media for fermented food production. This multi-functional capability makes the strain suitable for both probiotic and fermented milk applications.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The bacterium efficiently degrades xylans, producing organic acids and maintaining high viability, making it suitable for use as probiotics in fermented milk products and other food or drink products.
Implementation Method 1
The present invention relates to a bacterium belonging to the genus Bifidobacterium which has xylanase gene on the genome and can efficiently utilize xylans
Implementation Method 2
One of factors of probiotics influencing physiological functions of hosts is presumed to be that probiotics metabolize carbohydrate sources remaining in the digestive tract and produce short-chain fatty acids such as lactic acid and acetic acid
Data Source
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AI summary
Provided is a bacterium belonging to Bifidobacterium pseudocatenulatum which is capable of utilizing xylans and is excellent in viability in a milk component-containing medium. The present invention provides a bacterium belonging to Bifidobacterium pseudocatenulatum, the bacterium having xylanase gene on the genome and having higher viability in a milk component-containing medium than Bifidobacterium pseudocatenulatum YIT 11057 (NITE BP-02930).