Bacteria TRIZ Cases

Adjusting pH, NH4NO3, and lignin concentration boosts Erwinia sp. QL-Z3 degradation and LiP, MnP, and Lac enzyme activity.

Programmable media and environmental control enable growth of unculturable microbes and faster antimicrobial selection.

Engineered transaminase mutations broaden substrate scope and raise sitagliptin intermediate synthesis to 82% yield with 99% ee.

A Lactobacillus mali strain shortens steviol glycoside transglycosylation while improving taste, reducing off-notes, and increasing solubility.

Specific ilvB substitutions reduce AHAS feedback inhibition, raising L-isoleucine yield while suppressing norvaline and alpha-amino butyric acid.

Chromosomal integration of arginine-pathway genes in E. coli avoids plasmid loss and enables stable, high-yield L-arginine fermentation.

CDR alpha-chain mutations and an added disulfide bond boost TCR binding to FMNKFIYEI-HLA A0201 for more effective AFP tumor targeting.

Engineered genes and prpC deletions let microbes make PHBV from cheap single carbon sources, avoiding toxic organic acids.

Specific PhaC61-3 mutations enable fermentation of a viscous biodegradable PHA copolymer, expanding material options for degradable products.

Continuous Xanthobacter cultivation with hydrogen and CO2 raises protein productivity while keeping biomass suitable for safe food or feed use.

Adding sulfate during Bacillus fermentation precipitates or crystallizes unstable proteins, preserving cell viability and improving yield.

Perchlorate reductase and chlorite dismutase genes inserted into E. coli enable scalable perchlorate bioremediation beyond oxygen-limited bacteria.

Encapsulated Bacillus spores in a biodegradable polymer coating prevent MIC on submerged assets, then degrade away without toxic residue.

Controlled oxygen and acidic pH let engineered microbes convert ethylene glycol into glycolate with higher yield, lower buffer use, and simpler fermentation.

Biotinylated OMVs and avidin-linked antigens enable gentle APV coupling that preserves vesicle structure and antigen immunogenicity.

A three-bacteria composition promotes neural stem cell maturation, glial activation, and memory support through gut-brain modulation.

Modified Salmonella and a glycerol-phosphate medium raise Type III secretion titers while simplifying protein purification.

Engineered outer membrane binding and promoter control let bacteria sense cell-impermeable targets and trigger protein or RNA expression.

Bacterial strains replace heat-denatured plant myrosinase to convert glucosinolates into sulforaphane after cooking and in the gut.

Gut-established Lactococcus lactis GEN3033 secretes anticancer metabolites to inhibit cancer cell growth, enhance immunity, and support combination therapy.

A divided package keeps microorganisms separate from seed lubricant until planter use, preserving viability during storage and field delivery.

A CKDB001 probiotic composition targets poor NAFLD treatment compliance by reducing steatosis, liver inflammation, and cytokine levels.

Genetically boosted bacterioferritin improves microbial NOx-to-N2 reduction, cutting energy use, secondary waste, and N2O risk.

Attenuated Salmonella vectors co-express antigens and immune modulators to target tumors while limiting virulence and sustaining immune memory.

A minimal-media Methanothermobacter wolfeii strain converts CO2 and H2 to methane without organic supplements, lowering cultivation cost.

A glucomannan gel medium enables direct colony counting in large or turbid samples without filtration, heating, or divalent cations.

Longitudinal gut bacteria counts and blood LPS or LBP levels provide a simpler way to detect worsening or improvement in Parkinson's disease.

Static culture with tightly set pH and carbohydrate levels improves Bifidobacterium DNA uptake for genome editing and probiotic strain breeding.

A Bacillus coagulans strain replaces multi-strain probiotic blends by using prebiotics and glucose metabolism to lower blood glucose.

High-pressure homogenization breaks bacterial cells without mechanical contact, preserving active cell wall components and long-term stability.

Gas vesicles change cell acoustic contrast, enabling low-cost ultrasound sorting in microfluidic channels without complex FACS optics.

A dark-phase anaerobic shift in an alkaliphilic consortium releases intact phycocyanin, cutting extraction energy and process complexity.

An flgM-IL-21 secretion vector boosts therapeutic protein delivery in attenuated Salmonella while improving stability and anticancer activity.

Targeted gene and promoter mutations in Corynebacterium raise L-amino acid fermentation yield while avoiding full pathway redesign.

Adding at least 20 mM sulfate during Bacillus fermentation stabilizes native enzyme production by inducing precipitation and limiting proteolysis.

Selected L. coryniformis inoculation shortens pickle fermentation, degrades nitrite, and stabilizes flavor and batch quality.

Targeted GlyA mutations in coryneform bacteria raise L-glutamic acid accumulation while avoiding broader metabolic pathway control complexity.

Engineered Corynebacterium splits 1,4-butanediol synthesis into targeted reduction steps to raise yield and suppress by-products.

A peptide-linked fusion immunotoxin enables yeast extract in vitro synthesis with higher yield, lower cost, and stable cancer-cell targeting.

Targeted aceA mutation or knockout redirects glyoxylate-cycle flux to raise L-amino acid yield and lower fermentation cost.

A xylanase-bearing Bifidobacterium strain maintains viability in milk media while degrading xylans, supporting fermented milk probiotic use.

Engineered cells boost GlyA and methyltransferase activity to regenerate SAM from glycine or serine and speed methyl compound synthesis.

NaCl-adjusted peptone water and a cooled cascade impactor keep aerosol droplets in the 2.7-3.3 µm range for reliable fabric BFE testing.

A serine-383 substitution in acetyl-CoA hydrolase helps coryneform bacteria accumulate more L-glutamic acid during fermentation.

Soy peptone and yeast extract in fermentation and feed media raise Bacillus pectinase activity up to 4x for efficient production.

Sequential fermentation builds a block-structured PHA copolymer that improves ductility and anti-aging beyond random copolymers.

A CKDB001 and Bifidobacterium probiotic composition addresses poor NAFLD therapy compliance by modulating gut microbiota to reduce steatosis and inflammation.

By expressing SgTPS7 in E. coli, this case replaces low-yield plant extraction with enzymatic germacrene production at higher purity.

CO2 forms diamine carbonate intermediates during fermentation, enabling diamine isolation with fewer salt by-products and lower processing cost.

Specific substitutions at glucanase positions 53, 199, and 200 improve thermal stability while preserving glucan degradation in feed processing.