Glucanase Variant Amino Acid Substitutions for Thermal Tolerance

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Solution Overview

Problem

Existing glucanases lack thermal tolerance necessary for high-temperature processes in feed applications, hindering efficient nutrition intake in monogastric animals due to the absence of effective glucan degradation.

Innovation Solution

A modified polypeptide with substitutions at specific amino acid positions (53, 199, and 200) to enhance thermal tolerance, along with a polynucleotide encoding this polypeptide, a microorganism expressing it, and a vector for its production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If a glucanase enzyme is added to feed to enable glucan degradation, then nutrition intake efficiency is improved, but thermal tolerance is insufficient for high-temperature processing

Engineering Contradiction:

Improvenutrition intake efficiencyVSAvoidthermal tolerance

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent applies parameter changes by modifying specific amino acid residues (positions 53, 199, and 200) in the glucanase enzyme sequence. These substitutions alter the enzyme's thermal stability parameters while preserving its catalytic activity, enabling the enzyme to function effectively at high temperatures during feed processing without losing activity

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite enzyme system by combining the glucanase enzyme with specific amino acid substitutions that provide thermal stability. This modified enzyme composite maintains both the catalytic function for glucan degradation and the thermal resistance needed for high-temperature processing conditions

Inventive Principle:

Principle #40Composite materials

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The modified polypeptide exhibits improved thermal stability, enabling effective glucan degradation in various industrial fields, including feed, baking, and pulp bleaching.

Implementation Method 1

a modified polypeptide having glucanase activity... having glucanase activity... effective glucan degradation

Methodology Applied

Scientific EffectEnzymatic hydrolysis: Hydrolysis

Data Source

PatentUS20260028607A1Variant polypeptide having glucanase activity

Publication Date: 2026.01.29 KEMIN INDUSTRIES INC

AI summary

The present application relates to a glucanase variant.