Bioenergetic Bone Matrix Using PEMF and Shockwaves
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Solution Overview
Problem
Existing technologies face challenges in producing a safe allogeneic composition with viable cells for tissue regeneration, as they struggle to remove antigenic properties leading to inflammation and maintain the ability to modulate repair, while preserving the biologic function and lineage memory of stem cells.
Innovation Solution
A bioenergetic bone matrix derived from human cadaveric cortical bone is manufactured through mechanical, electrical, and magnetic energy transfers, enhancing osteoinductivity and osteoconductivity by processing methods such as PEMF, shockwave, and negative pressure to expose morphogenetic proteins and growth factors, creating a biologically energized composition.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If stem cells are isolated and purified to achieve safe allogeneic composition, then antigenic properties are reduced, but the quantity of viable cells and biologic function is degraded
Solution Approach 1:
The patent extracts and removes antigenic properties and inflammatory factors from bone marrow through specialized processing, isolating only the beneficial stromal cell components while discarding harmful elements, thereby achieving safety without compromising cell quantity or viability
Solution Approach 2:
The patent applies changes in physical and chemical parameters during processing to selectively preserve viable stem cells while removing antigenic properties, using controlled conditions that maintain cell viability throughout the purification process
2Object-affected harmful factors
If stem cells are purified to remove antigenic properties, then inflammation is reduced, but the ability to modulate repair is compromised
Solution Approach 1:
The patent selectively extracts and removes only the harmful antigenic properties and inflammatory factors while preserving the essential repair-modulating capabilities of stromal cells, achieving a purified composition that maintains therapeutic functionality
Solution Approach 2:
The patent applies different processing conditions to different components of bone marrow, selectively preserving the repair-modulating properties of stromal cells while removing inflammatory factors, thereby achieving localized purification that maintains functional versatility
3Reliability
If energy transfer methods are applied to enhance bioenergetics, then osteoinductivity and osteoconductivity are improved, but processing complexity increases
Solution Approach 1:
The patent replaces complex mechanical processing systems with energy transfer methods (electromagnetic, acoustic, mechanical vibration) to enhance bioenergetics, using non-mechanical or simplified mechanical means to achieve improved osteoinductivity and osteoconductivity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The bioenergetic bone matrix supports regenerative applications by sustaining viability and metabolic support, promoting new tissue formation, and integrating host and donor tissues effectively.
Implementation Method 1
The methods which promote these effects are PEMF (Pulsed Electro-Magnetic Fields)
Implementation Method 2
Examples of tissue response to pressure waves, electric fields, magnetic fields, pressure variations, and ion streaming induction with pH are known in the literature
Implementation Method 3
ion streaming induction with pH are known in the literature
Data Source
AI summary
A biological composition has a mixture of mechanically selected allogeneic biologic material derived from bone marrow. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture including non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts. The mixture is compatible with biologic function.