Biological Data Storage via Composite DNA Sequences

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Solution Overview

Problem

Current DNA data storage technologies face limitations in information storage density, mutation rates, and biocompatibility, with existing algorithms like binary and ternary conversion methods not fully addressing issues of error correction and compatibility when integrating data DNA into biological organisms.

Innovation Solution

The method involves converting data into a set of single-stranded DNA short sequences, incorporating an indexDNA, dataDNA, and correctionDNA modules to prevent mutations and ensure biocompatibility, with an encryption unit for secure data storage and retrieval, allowing for efficient storage in biological organisms like cells.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If binary or ternary conversion algorithms are used to convert data to DNA sequences, then data storage is achieved, but information storage density is limited and mutation rates increase

Engineering Contradiction:
Improveinformation storage densityVSAvoidmutation rate
Core Design Contradiction:
Quantity of substanceVSReliability

Solution Approach 1:

The patent applies a composite coding structure combining multiple DNA sequences with different functions (data storage sequences, error correction sequences, and position information sequences) to create a robust data storage system that achieves high density while maintaining reliability through diversified sequence composition

Inventive Principle:
Principle #40Composite materials

Solution Approach 2:

The patent divides data into multiple data conversion units and encodes each unit as a separate DNA sequence, allowing parallel storage and reducing the impact of mutations on overall data integrity through segmented error correction capabilities

Inventive Principle:
Principle #1Segmentation

2Adaptability or versatility

If data DNA is integrated into carrier cell genomes for biological storage, then biocompatibility is improved, but the passaging process may introduce mutations into data DNA sequences

Engineering Contradiction:
ImprovebiocompatibilityVSAvoiddata DNA mutation prevention
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent incorporates error correction sequences and position information sequences into the DNA structure before biological storage, providing preemptive protection against mutations that may occur during carrier cell passaging and enabling post-retrieval error detection and correction

Inventive Principle:
Principle #11Beforehand cushioning (Prior cushioning)

Solution Approach 2:

The patent uses position information sequences and error correction codes that provide feedback mechanisms to detect and correct mutations after DNA retrieval from biological carriers, ensuring data integrity despite potential mutations during storage

Inventive Principle:
Principle #23Feedback

3Reliability

If quadruple redundancy mechanism is used to prevent errors in DNA synthesis and sequencing, then error correction capability is improved, but conversion efficiency decreases and synthesis cost increases four times

Engineering Contradiction:
Improveerror correction capabilityVSAvoidconversion efficiency
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent implements selective redundancy by adding error correction sequences and position information sequences only where necessary, rather than quadrupling all data sequences, achieving adequate error protection while maintaining reasonable conversion efficiency and reducing synthesis costs

Inventive Principle:
Principle #16Partial or excessive action

Data Source

PatentEP3509018B1Method for biologically storing and restoring data
Publication Date: 2023.10.18 TSINGHUA UNIVERSITY
  • EP3509018B1 patent drawingFigure 1
  • EP3509018B1 patent drawingFigure 2
  • EP3509018B1 patent drawingFigure 3a~4f

AI summary

The present invention relates to a method, device and software product for converting data into a data DNA sequence and restoring the DNA sequence library into raw data, and a storage medium for storing the software product. The method for converting data into a data DNA sequence comprises: dividing data into one or more data conversion units, providing a binary sequence for each data conversion unit, and converting each data conversion unit into a data DNA sequence according to a dataDNA sequence conversion rule, thus acquiring a data DNA sequence library, which makes biological storage of data in vivo possible by constructing a data DNA library.