Bivalve Ether Phospholipid Extraction via Solvent Partitioning
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Solution Overview
Problem
Current methods for producing plasmalogens-type glycerophospholipids, particularly from chicken tissues, are not sufficient in terms of effectiveness for treating and improving brain diseases, metabolic diseases, infectious diseases, and immune disorders, and lack efficiency in large-scale production.
Innovation Solution
A method involving the processing of bivalve tissues with a water-soluble ketone solvent, followed by extraction with a hydrophobic organic solvent and reaction with phospholipase A1 to obtain purified ether phospholipids, which are then separated using solvent partitioning.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If plasmalogens-type glycerophospholipids are produced from chicken tissues using conventional methods, then production can be achieved on a large scale, but the effectiveness for treating brain diseases and other disorders is insufficient
Solution Approach 1:
The invention changes the source material parameter from chicken tissues to bivalve tissues, which fundamentally alters the composition and effectiveness of the produced plasmalogens. This parameter change enables both high effectiveness for treating brain diseases and efficient large-scale production, resolving the contradiction between therapeutic reliability and manufacturing ease
2Manufacturing precision
If ether phospholipids are extracted from bivalve tissues using multiple extraction steps, then purity and effectiveness are improved, but production complexity increases
Solution Approach 1:
The invention employs a systematic extraction process that selectively removes ether phospholipids from bivalve tissues through sequential steps: water-soluble ketone solvent extraction to remove neutral fats, followed by hydrophobic organic solvent extraction to isolate glycerophospholipids. This targeted extraction approach achieves high purity while maintaining manageable process complexity
Solution Approach 2:
The production process is divided into distinct sequential stages: (A) neutral fat removal using water-soluble ketone solvent, (B) glycerophospholipid extraction using hydrophobic organic solvent, and (C) enzymatic decomposition with phospholipase A1 followed by solvent partitioning. This segmentation allows each step to be optimized independently, achieving high purity without excessive overall complexity
3Ease of manufacture
If conventional extraction methods are used for plasmalogens from chicken breast, then production is easier compared to chicken skin, but the therapeutic effects for brain diseases and metabolic diseases remain insufficient
Solution Approach 1:
The invention changes the fundamental parameter of source tissue from chicken breast to bivalve tissues. This parameter change provides both the ease of large-scale production characteristic of chicken breast and the superior therapeutic effectiveness associated with plasmalogens from bivalve sources, simultaneously resolving both concerns
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The resulting ether phospholipids from bivalve tissues exhibit superior effects in treating and improving brain diseases, metabolic diseases, infectious diseases, and immune disorders, and can be produced on a massive scale with ease.
Implementation Method 1
a step of processing total lipids of bivalve tissues with a water-soluble ketone solvent to get bivalve phospholipids without neutral fat
Implementation Method 2
a step of providing extraction processing from the bivalve phospholipids without neutral fat obtained by the step (A) with a hydrophobic organic solvent to get glycerophospholipids
Implementation Method 3
a step of reacting the glycerophospholipids obtained by the step (B) with phospholipase A1 to decompose mixed diacyl-glycerophospholipids
Data Source
Figure 1(a)~2(b)
Figure 3(a)~3(c)
AI summary
[PROBLEM TO BE SOLVED] It enables any superior ether phospholipids to the conventional ones and a method for producing the same in an easy manner on a massive scale, in light of effects of treating and improving brain diseases such as Alzheimer's disease, Parkinson disease, depression and schizophrenia, metabolic diseases such as diabetes, various infectious diseases, and immune disorders. [SOLUTION] Ether phospholipids are obtained from bivalve tissues such as clams and corbicula by extraction processing. The ether phospholipids exhibit significantly superior effects of the above as compared to the conventional ether phospholipids derived from chicken tissues.