Candida tropicalis Am2525 Fermentation for Decane-Resistant Decanedioic Acid
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Solution Overview
Problem
Current methods for producing decanedioic acid, such as chemical synthesis from castor oil, are environmentally harmful and costly due to high temperatures and toxic reagents, while biological processes face issues like low thallus concentration, long fermentation periods, and low conversion efficiency, leading to high production costs.
Innovation Solution
A Candida tropicalis strain, Am2525, is developed through mutation breeding using atmospheric and room temperature plasma (ARTP) and ultraviolet irradiation, enhancing its resistance to toxic substrates like decane, allowing for efficient fermentation of C10 n-alkanes to decanedioic acid with improved conversion efficiency and reduced production costs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical synthesis from castor oil is used to produce decanedioic acid, then the production process can achieve high conversion, but the process becomes environmentally harmful and costly due to high temperatures and toxic reagents
Solution Approach 1:
The patent replaces the chemical synthesis method (mechanical/chemical system) with a biological fermentation system using Candida tropicalis Am2525. The strain metabolizes n-alkanes through enzymatic pathways to produce decanedioic acid, eliminating the need for high-temperature reactions and toxic reagents like phenol and o-cresol, thus resolving the environmental pollution issue while maintaining production efficiency
Solution Approach 2:
The patent changes the fundamental parameters of the production system from chemical (high temperature 250-270°C, toxic catalysts) to biological (mild fermentation conditions, enzymatic catalysis). The strain Am2525 operates at ambient temperatures and pH levels, transforming the reaction conditions to eliminate harmful factors while achieving comparable or superior conversion rates
2Object-affected harmful factors
If biological fermentation process is used to produce decanedioic acid, then the process becomes environmentally friendly, but the thallus concentration remains low and fermentation period becomes long
Solution Approach 1:
The patent applies preliminary action by pre-adapting and selecting the Candida tropicalis strain through mutation breeding (ARTP and UV irradiation) to enhance its tolerance to n-alkane substrates and improve its metabolic efficiency. This preliminary strain optimization enables the fermentation process to achieve higher thallus concentration and faster growth rates, addressing the low productivity issue while maintaining environmental friendliness
Solution Approach 2:
The patent optimizes fermentation parameters including substrate concentration, pH, temperature, and aeration to create optimal growth conditions for strain Am2525. These parameter adjustments significantly improve thallus concentration and reduce fermentation time compared to conventional biological processes, while maintaining the environmentally friendly nature of the system
3Productivity
If decane is used as substrate for fermentation, then the substrate can be converted to decanedioic acid, but the toxic effects of decane inhibit the growth of microorganisms
Solution Approach 1:
The patent applies preliminary action by pre-exposing the Candida tropicalis strain to increasing concentrations of n-alkanes during the adaptation and selection process. This gradual exposure induces the development of toxic resistance mechanisms, enabling the strain to tolerate and efficiently convert decane substrates without significant growth inhibition, thus resolving the contradiction between substrate conversion and microorganism growth
Solution Approach 2:
The patent transforms the harmful toxic effect of decane on microorganisms into a beneficial selective pressure during strain development. The toxic substrate becomes a tool for selecting and optimizing strains with enhanced resistance and conversion capabilities. Strain Am2525 has been specifically selected to thrive in the presence of decane, converting the harmful substance into a valuable substrate while maintaining robust growth
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Candida tropicalis Am2525 strain achieves higher thallus concentration and substrate conversion, reducing production costs and simplifying the fermentation process, with conversion efficiencies over 35% and potential for scalable industrial application.
Implementation Method 1
a method for producing a long-chain dicarboxylic acid via fermentation by using the strain
Implementation Method 2
during the possible transformation pathways in the fermentation, are oxidized in multiple steps by thalli to the target product decanedioic acid
Implementation Method 3
The strain provided in the present disclosure is Candida tropicalis Am2525, which is screened out by using the strain Candida tropicalis CAT N145 of producing mixed dicarboxylic acids through oxidizing n-alkanes
Data Source
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Figure 3
AI summary
Provided are a Candida tropicalis strain Am2525, with the preservation number thereof being CCTCC NO: M 2019419, and a method for producing long-chain dicarboxylic acids by means of fermenting the strain. The method for producing the long-chain dicarboxylic acids comprises preparing a seed solution by means of the Candida tropicalis strain Am2525 and producing the long-chain dicarboxylic acids via fermentation of the seed solution. Compared with the parent, the Candida tropicalis strain Am2525 has an enhanced resistance to the toxicity of a substrate decane, improves the productivity of long-chain dicarboxylic acids, reduces the cost of production, subsequent separation and purification are simple, and the fermentation production process is easy to implement on a large scale.