Cell Surface Nucleic Acid Control for Non-Genetic Cell Regulation
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Solution Overview
Problem
Existing methods for altering cell properties often rely on mutagens, gene introduction, or environmental changes, which can be invasive and have limitations.
Innovation Solution
The use of nucleic acid inactivating products and nucleases to manage cell properties without genetic modification, through treatments like Cut-D, Cut-R, Zero-D, and Zero-R cycles, altering nucleic acid molecules associated with cell surfaces (NAMACS and NAMACS-ANA) to control cell behavior and functions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If mutagens or gene introduction methods are used to alter cell properties, then the cell properties can be changed, but the method becomes invasive and complex
Solution Approach 1:
The patent extracts and targets specific nucleic acid molecules (NAMACS and NAMACS-ANA) that are associated with cell surfaces and responsible for cell properties. By using nucleases to specifically degrade these external nucleic acid molecules rather than introducing foreign genes or using mutagens throughout the cell, the method achieves precise cell property alteration without the complexity of genetic engineering tools.
Solution Approach 2:
The patent introduces nucleic acid inactivating products (nucleases) as intermediaries that selectively degrade NAMACS and NAMACS-ANA. These intermediaries mediate between the desired cell property change and the cell itself, allowing controlled alteration of cell properties through targeted degradation of specific external nucleic acid molecules without direct genetic modification.
2Manufacturing precision
If gene editing tools are introduced into cells, then genetic structure can be changed, but the process requires special introduction methods and becomes more invasive
Solution Approach 1:
The patent converts the potentially harmful effect of external nucleic acid molecules (NAMACS and NAMACS-ANA) that mediate unwanted cell properties into a benefit by selectively degrading them. Instead of trying to modify the cell's internal genome, the method targets and degrades the external nucleic acid molecules that are causing the undesired effects, thereby eliminating the harm while achieving the desired cell property change.
3Adaptability or versatility
If environmental conditions are changed to control cell behavior, then cell properties can be regulated, but the method lacks precision and requires complex environmental control
Solution Approach 1:
The patent uses nucleic acid inactivating products as specific intermediaries that directly target NAMACS and NAMACS-ANA molecules. This provides precise control over cell behavior by specifically degrading the nucleic acid molecules responsible for particular cell properties, rather than requiring broad environmental changes that affect all cellular processes.
4Manufacturing precision
If multiple treatment cycles are applied to achieve desired cell state, then cell properties can be precisely regulated, but the treatment time increases
Solution Approach 1:
The patent employs periodic treatment cycles where cells are treated with nucleic acid inactivating products, then allowed to grow or differentiate, and treated again in subsequent cycles. This periodic application of treatments achieves precise control over cell state and properties by progressively modifying the cell population through repeated, controlled interventions rather than requiring continuous treatment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for precise regulation of cell properties and functions, including disease management, gene switching, and environmental adaptation, without genetic modification, enhancing treatment efficacy and efficiency.
Implementation Method 1
treatments like Cut-D, Cut-R, Zero-D, and Zero-R cycles, altering nucleic acid molecules associated with cell surfaces (NAMACS and NAMACS-ANA)
Data Source
AI summary
The invention relates to medicine, biology, veterinary, pharmacology diagnostics, agriculture, ecology, meteorology, seismology, construction biotechnology, biomanufacturing and provided herein are products and methods for managing cells behavior, memory of cells and erasure of cell memory. The present invention describes products and methods that, unlike the known ones, make it possible to control the properties of cells and organisms without the use of mutagens and/or the special introduction of genes and/or use of specific gene tools and/or changing its environmental conditions.