Hydrolases TRIZ Cases

Terminal adaptor-modified donor templates improve HDR and HITI precision, especially for longer inserts that usually lose gene editing efficiency.

Water-assisted lipase hydrolysis lowers mono- and diglycerides in oil while avoiding substantial triglyceride interesterification and extra refining.

Herbicide-resistance genes in mitochondria enable direct selection of transformed plant or algal cells, simplifying stable genome editing.

An engineered CRISPR-Tn7 transposon inserts donor DNA near a gRNA target without double-strand breaks, improving integration in non-dividing cells.

Nucleases target cell-surface nucleic acids to regulate cell behavior and memory without mutagens, gene insertion, or environmental shifts.

Engineered Type I-F3 CRISPR with Tn7-like transposons improves RNA-guided DNA integration efficiency and target specificity across bacteria.

Targeted PSTOL1 editing stabilizes the protein to build steeper, denser roots that improve nutrient uptake, stress resilience, and yield.

Optimized in-house IVT enzymes improve mRNA yield, purity, and cell transfection by refining expression vectors and purification steps.

Donor-derived cell-free DNA from blood, plasma, or urine enables earlier non-invasive xenotransplant rejection detection than biopsy.

Barcode-guided combinatorial assembly links genetic components without extra junction sequences, speeding Cas9 variant screening with lower off-target activity.

Dual Chryseobacterium enzymes pre-treat plant proteins to raise essential amino acid release and improve foam stability and emulsifiability.

CRISPR editing of the CaAIBZ1 gene enables bi-allelic hot pepper lines with stronger drought survival and recovery under water stress.

Specific GFAP binding enables undiluted serum detection from 10 to 50,000 pg/mL, supporting rapid point-of-care TBI assessment.

BridgeRNA guides IS110 transposases to specific DNA sites, enabling programmable insertion, inversion, and excision without complex protein engineering.

Heat, pressure, and myrosinase lower glucosinolates in oilseed meal while preserving protein quality for feed use.

Barcoded linear dsDNA libraries and sequencing reduce assay noise while revealing off-target binding, modification, and cleavage sites.

Using the Ces1d 5' UTR, this case boosts cardiac and hepatic modRNA translation from one dose while reducing immune activation.

Pre-enriched barcoded DNA libraries improve off-target site detection by avoiding genomic noise and sequence bias in base-editing assays.

Host cell modulation of Hsc/Hsp70, vacuolar H+ ATPase, and CDK2 boosts rAAV assembly titer and improves vector quality.

Improved CRISPR/Cas9 and Cpf1 knock-in systems raise HDR efficiency in human stem cells while reducing off-target DNA insertion.

Blocking-region probes and auxiliary signaling enable single-tube high-plex PCR detection while reducing primer-dimer false positives.

Targeted amino acid substitutions raise heterologous lipase expression without chaperone co-expression, lowering production cost for industrial use.

A replicon-linked base editing vector sustains sgRNA expression to silence multiple genes while avoiding double-strand breaks.

Targeted esterase mutations improve thermal stability and catalytic efficiency for polyester hydrolysis across wide pH and 37°C to 70°C.

Machine-learning prediction of interacting gene endophenotypes helps guide genome editing before mature plant traits develop.

Targeted DNA and RNA inactivation alters NAMACS, NAMACS-ANA, and TEZRs to regulate cell behavior without gene editing or environmental shifts.

Segmented tiny guide RNA pairing preserves AGO binding while exposing a non-pairing region that improves target nucleic acid cleavage.

SHMT2-edited mammalian cells create glycine auxotrophy for dual selection, enabling multi-vector protein expression with high viability.

Modular gene cassettes in pigs regulate complement, coagulation, and innate immunity to improve xenograft survival and tolerance.

Chemically modified and truncated AsCpf1 crRNAs enable AT-rich DNA targeting while shortening guide RNA and preserving editing activity.

Protein deamidase added around alkaline extraction lowers plant protein isoelectric point to improve solubility and food functionality.

Targeted amino acid changes in cold-water proteases help laundry products break down protein stains and improve freshness in low-temperature washes.

RNA-guided genome editing creates dominant plant alleles by deleting or inverting targeted chromosomal regions to alter gene expression.

Fluorinated polyamine carriers improve membrane affinity to boost biomolecule transfection while preserving cell viability in hard-to-balance delivery systems.

Recombinant bacteria use YbgC and acyl-CoA synthetase to make natural lactones at neutral pH without chemical conversion steps.

Destabilization-domain guide adaptors make CRISPR activity dose- and time-switchable, reducing off-target DNA cleavage while preserving editing control.

Enzymatic glucosylation, oxidation, and deamination map 5mC and 5hmC on intact long DNA while avoiding fragmentation and sequence bias.

A marine heat-labile 3′-5′ exonuclease creates 5′ overhangs at room temperature, simplifying multi-fragment DNA assembly and cleanup.

A genome-integrated naked FRB domain preserves rapamycin-induced CISC signaling while attenuating host cell growth inhibition and toxicity.

Pollen from a transformed haploid inducer delivers CRISPR machinery during fertilization, enabling edited haploid progeny without tissue culture.

A CBD-His dual tag lets Serratia marcescens nuclease digest nucleic acids, then be immobilized and removed to avoid polymerase interference.

Site-specific landing pad recombination with split selectable markers cuts clone screening and speeds stable producer cell line generation.

Targeted guide RNA and endonuclease excision removes selectable markers and rearrangements from INIR6 maize while preserving insect resistance traits.

CRISPR-Cas9 deletion of CD163 exon 7 blocks PRRSv uptake in pigs, reducing viral load and avoiding vaccine failure across diverse strains.

A glycine-to-aspartic acid mutation at position 10 raises phospholipase C activity for vegetable oil degumming without reducing unit degumming ability.

Engineered meganucleases cut dystrophin introns to remove exons 45-55, restore the reading frame, and enable lasting DMD correction.